A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples.

Introduction: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis.

Methods: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were used in the second reaction.