Saccharification of acid-alkali pretreated sugarcane bagasse using immobilized enzymes from .

Unlabelled: In this study, a mild-temperature two-step dilute acid and alkaline pretreatment (DA-AL) process was developed to generate highly digestible cellulose pulp from sugarcane bagasse for producing fermentable sugars by novel thermophilic cellulases derived from SC 04. First, DA pretreatment of sugarcane bagasse at 2% (w/v) HSO and 121 °C for 71 min, followed by AL pretreatment at 2.2% (w/v) NaOH and 110 °C for 100 min led to the pulp containing 86% cellulose.