Evaluation of Volumetric Absorptive Microsampling and Mass Spectrometry Data-Independent Acquisition of Hemoglobin-Related Clinical Markers.

Data-independent acquisition (DIA) allows comprehensive proteome coverage, while it also potentially works as a unified protocol to determine a multitude of proteins found in blood. Because of its high specificity, mass spectrometry may greatly reduce the interference observed in other assays to evaluate blood markers. Here, we combined DIA with volumetric absorptive microsampling (VAMS) and automated proteomics sample processing in a platform to assess clinical markers.

Effects of cigarette smoke on color, roughness and gloss of high-viscosity bulk-fill resin composites.

Purpose: To evaluate the effect of cigarette smoke (CS) on color, roughness and gloss of bulk-fill resin composites.

Methods: Resin discs (10 x 2 mm) were made for resin composites (n= 10) : Filtek Z250XT (control), Filtek One Bulk Fill (FOBF), Tetric N-Ceram Bulk-Fill (TBF) and Aura Bulk-Fill (ABF). The color ( ΔL*, Δa*, Δb*, ΔE and ΔE00), roughness (Ra) and gloss analyses were performed at the baseline and after CS exposure (10 packs of cigarettes - Marlboro Red).

Children's satisfaction and dissatisfaction with pain management in a Pediatric Emergency Department.

Objective: To understand children's satisfaction and dissatisfaction with pain management in the pediatric emergency department.

Method: Exploratory-descriptive qualitative study performed with children between 6 and 12 years of age and use of semi-structured interviews and thematic analysis of data.

Results: Nineteen children were interviewed.

Effect of Heavy Metals Contamination from Cigarette Smoke on Sound and Caries-Like Enamel.

In this study, we sought to evaluate the influence of cigarette smoke and pH cycling on the chemical composition and surface/cross-sectional enamel microhardness. A total of 40 dental blocks obtained from bovine incisors were divided into four groups (n=10): no treatment (control); exposure to cigarette smoke (CS); exposure to pH cycling (PC); and exposure to cigarette smoke and pH cycling (CS-PC). The samples were analyzed by synchrotron radiation micro X-ray fluorescence, bench mode X-ray fluorescence, as well as surface microhardness (SMH) and cross-sectional microhardness (CSMH) testing.

Influence of pre-heating and ceramic thickness on physical properties of luting agents.

Purpose: The objective of this in vitro study was to evaluate the influence of ceramic thickness and pre-heating of luting agents on their physical properties.

Materials And Methods: The materials RelyX Arc, RelyX Ultimate, RelyX Veneer, and Filtek Z350 Flow were handled at different temperatures (23°C or 54°C), inserted into matrix, and photoactivated through ceramic disks (0.75 mm or 1.

Influence of Enamel Thickness on Bleaching Efficacy: An In-Depth Color Analysis.

This study evaluated the influence of different enamel thicknesses and bleaching agents on treatment efficacy in-depth by spectrophotometry color analysis. Eighty bovine dental fragments were previously stained in black tea solution and randomly assigned into eight groups (n=10), 1.75mm dentin thickness and different enamel thicknesses as follows: 0.

Evaluation of physical properties of a nanocomposite after aging, bleaching and staining.

Purpose: The objective of the in vitro study was to assess the effect accelerated artificial aging, bleaching agents and coffee staining on the color, gloss, roughness and microhardness of a nanocomposite.

Materials And Methods: 120 (7 × 2 mm) were prepared and randomly divided into 2 groups (n = 60) depending on the aging. Each group was further subdivided into 6 subgroups (n = 10) according to the bleaching and staining as follows: 10% carbamide peroxide (10% CP), 10% CP + staining, 35% hydrogen peroxide (35% HP), 35% HP + staining, without bleaching treatment (WB) and WB + staining.

In vitro evaluation of the effect of different polishing techniques on the surface roughness of composite resins submitted to at-home and in-office bleaching procedures.

Objective: Bleaching agents may affect the properties of dental materials. The aim of this study was to evaluate the effect of different polishing techniques on the surface roughness of composite resins submitted to the at-home and in-office bleaching treatment.

Materials And Methods: Disc-shaped specimens were carried out of nanofilled and microhybrid composites (n = 10).

Adhesive Restorations as An Esthetic Solution in Dentinogenesis Imperfecta.

Loss of tooth structure is the main sequela of dentinogenesis imperfecta (DI). Due to severe enamel attrition, patients with DI often present with esthetic, occlusal, endodontic, and speech complications. Therefore, an interdisciplinary approach, divided into separate clinical steps, should be developed to provide comprehensive dental rehabilitation.

Effect of 7.5% hydrogen peroxide containing remineralizing agents on hardness, color change, roughness and micromorphology of human enamel.

Purpose: To determine the microhardness, color change, surface roughness, and micromorphology of tooth enamel submitted to bleaching treatment with 7.5% hydrogen peroxide (HP) with added calcium, amorphous calcium phosphate, sodium fluoride (NaF), and hydroxyapatite (HA).

Methods: 80 enamel slabs were used (n = 10).

Enamel morphology after microabrasion with experimental compounds.

Background: Enamel microabrasion is an esthetic treatment for removing superficial stains or defects of enamel.

Aim: This study evaluated the roughness after enamel microabrasion using experimental microabrasive systems.

Materials And Methods: One hundred and ten samples (5 × 5 mm) were obtained from bovine incisors and divided into 11 groups (n = 10) in accordance with the treatment: Microabrasion using 6.

In vitro effects of hydrogen peroxide combined with different activators for the in-office bleaching technique on enamel.

Objective: The aim of this study was to evaluate the alteration of human enamel bleached with high concentrations of hydrogen peroxide associated with different activators.

Materials And Methods: Fifty enamel/dentin blocks (4 × 4 mm) were obtained from human third molars and randomized divided according to the bleaching procedure (n = 10): G1 = 35% hydrogen peroxide (HP - Whiteness HP Maxx); G2 = HP + Halogen lamp (HL); G3 = HP + 7% sodium bicarbonate (SB); G4 = HP + 20% sodium hydroxide (SH); and G5 = 38% hydrogen peroxide (OXB - Opalescence Xtra Boost). The bleaching treatments were performed in three sessions with a 7-day interval between them.

Enamel microabrasion: An overview of clinical and scientific considerations.

Superficial stains and irregularities of the enamel are generally what prompt patients to seek dental intervention to improve their smile. These stains or defects may be due to hypoplasia, amelogenesis imperfecta, mineralized white spots, or fluorosis, for which enamel microabrasion is primarily indicated. Enamel microabrasion involves the use of acidic and abrasive agents, such as with 37% phosphoric acid and pumice or 6% hydrochloric acid and silica, applied to the altered enamel surface with mechanical pressure from a rubber cup coupled to a rotatory mandrel of a low-rotation micromotor.

Influence of successive light-activation on degree of conversion and Knoop hardness of the first layered composite increment.

Purpose: To evaluate the influence of light-activation of second, third and fourth increments on degree of conversion (DC) and microhardness (KHN) of the top (T) and bottom (B) surface of the first increment.

Materials And Methods: Forty samples (n = 5) were prepared. In groups 1-4, after each increment light-activation (multiple irradiation), T and B of the first increment were measured in DC and KHN.

Effect of light curing protocol on degree of conversion of composites.

Objective: To evaluate the degree of conversion (DC) of two light-cured composites with different protocols of light curing.

Materials And Methods: One hundred and ninety two specimens (n = 8) were prepared (5 mm × 2 mm) according to experimental groups: two composite resins (Filtek Supreme and four seasons); three light curing protocols [20 s with the tip of the light curing unit (LCU) device touching composite surface (C); 20 s with the tip of the LCU at 8 mm distant from composite surface (D); and tip of the LCU at 8 mm distant from composite surface and polymerization time required to obtain a radiant exposure of 16 J/cm(2) (DS)]. Four LCUs (Bluephase 16i, Ultralume LED 5, XL 3000 and Optilux 501C) were used.

Influence of light-activation protocol on methacrylate resin-composite evaluated by dynamic mechanical analysis and degree of conversion.

The aim of this study was to evaluate the degree of conversion (DC) and to identify the viscoelastic properties: storage modulus (E'), loss modulus (E"), tangent delta (tan δ), and glass transition temperature (T g ) of a microhybrid resin-composite light-activated by three different protocols. A Filtek Z250 (3 M ESPE) shade A3 was inserted in a Teflon mold (21 mm × 5 mm × 1 mm for viscoelastic properties; and 5 mm × 1 mm for DC) and light-activated according to the following light-activation protocols: (S) 1,000 mW/cm(2) × 19 s, (HP) 1,400 mW/cm(2) × 14 s, and (PE) 3,200 mW/cm(2) × 6 s, all set up to deliver 19 J/cm(2). Viscoelastic properties was assessed by dynamic mechanical analysis (DMA) (n = 3), performed in single cantilever clamped mode.

Effect of different monomer-based composites and acid etching pre-treatment of enamel on the microleakage using self-etch adhesives systems.

Objective: To evaluate quantitatively the marginal microleakage of restorations carried out with self-etching adhesives with or without prior phosphoric enamel acid etching of silorane or methacrylate resin-based composite restorations subjected to thermal cycling.

Materials And Methods: Forty cavities were prepared at the proximal surface of bovine incisors and randomly divided according to the etching of the enamel and restorative system used. The groups were restored with methacrylate [Adper SE Plus adhesive (3M ESPE) + Filtek Z250 (3M ESPE)] or silorane [Filtek LS adhesive (3M ESPE) + Filtek LS composite (3M ESPE)] restorative systems, light-cured using a LED unit (Bluephase 16i, Vivadent).

Effect of energy density and delay time on the degree of conversion and Knoop microhardness of a dual resin cement.

Aim: In the present study, we evaluated the influence of the photo-curing delay time and energy density on the degree of conversion and the Knoop microhardness of a resin cement.

Methods: Seventy-eight samples were assigned to 13 groups (n = 6), one of which received no light curing (control). The samples were made of a dual-cured resin cement (RelyX ARC) with the aid of a Teflon matrix, submitted to one of the following energy densities (J/cm²): 7, 14, 20, and 28.

Influence of surface treatments on enamel susceptibility to staining by cigarette smoke.

Objectives: The purpose of this study was to evaluate the influence of remineralizing agents, including artificial saliva, neutral fluoride, and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), on the susceptibility of bleached enamel to staining by cigarette smoke.

Study Design: Fifty bovine enamel blocks were randomly divided into five groups (n = 10): G1- bleaching; G2- bleaching and immersion in artificial saliva; G3- bleaching and application of CPP-ACP; G4- bleaching and application of neutral fluoride; and G5- untreated (Control). Teeth were bleached with 35% hydrogen peroxide and treated with the appropriate remineralizing agent.

Effectiveness of dental bleaching in depth after using different bleaching agents.

Objectives: This study evaluated the effectiveness of low- and high-concentration bleaching agents on enamel and deep dentin.

Study Design: Stained bovine incisors fragments were randomized placed into 10 groups (n=5), according to the sample thicknesses (2.0 mm or 3.

Efficacy of tooth bleaching with the prior application of a desensitizing agent.

Aim: In the present study, we evaluated the efficacy of bleaching on enamel and opposite dentin surfaces using 35% hydrogen peroxide (HP) with the prior application of a desensitizing agent.

Methods: Thirty bovine dental fragments, with thicknesses of 1 mm enamel and 1.75 mm dentin, were stained in a solution of black tea.

Effect of polymerization mode and time of adhesive system on microleakage in composite resin restorations.

Aim: To quantify the microleakage on restorations carried out with a two-step etch-and-rise adhesive system and nanofilled composite resin after thermal and mechanical loading cycling.

Methods: Ninety cavities were prepared on proximal surfaces of incisive teeth, and were randomly divided according to the photoactivation time (10, 20, or 30 s) and light-curing mode (Quartz Tungsten Halogen (QTH) lamps, 450 mW/cm(2); Light Emitting Diode (LED) second generation, 1100 mW/cm(2); or LED third generation, 700 mW/cm(2)) of an adhesive system (n = 10). Following restorative procedures and thermal and mechanical loading cycling, the samples were immersed in Methylene Blue for 2 h.

Influence of flowable composite and restorative technique on microleakage of class II restorations.

Aim: The aim of the present study was to evaluate the microleakage in class II cavities restored with dental composite by varying the thickness of two flowable composite resins and the restorative technique.

Methods: A total of 130 cavities were prepared on proximal surfaces of bovine teeth and randomly divided in 13 groups. Twelve experimental groups were performed using flowable composite resin as the liner, and they were divided according to the type of flowable composite resin (Filtek Z350 Flow-Z350F and SureFil SDR Flow-SUR), the thickness of the flowable composite (0.

Esthetic and functional rehabilitation via reattachment of dental fragments.

Anterior teeth fractures are frequently encountered in clinical practice. The reattachment of dental fragments, if possible, is a conservative and effective technique, which is utilized in this case. This case presentation concerns an 18-year-old male patient with a root-crown fracture of the left maxillary central incisor, which involves the periodontal biologic space.

Influence of coffee and red wine on tooth color during and after bleaching.

Objective: The aim of this study was to evaluate the influence of coffee and red wine staining on tooth color during and after bleaching.

Materials And Methods: Blocks obtained from human molars were divided into 11 groups (n = 5) in accordance with the bleaching treatment-peroxide carbamide 10%, 15% or 20%-and in accordance with the stain therapy-coffee, wine or without staining (control). Color change analysis was performed by photo-reflectance using a spectrophotometer, during (3-times/week) and after (7, 15 and 30 days) the bleaching treatment.