Sesquiterpenoid-rich Java Ginger rhizome extract prompts autophagic cell death in cervical cancer cell SiHa mainly by modulating cellular redox homeostasis.

Unlabelled: Java Ginger or Curcuma zanthorrhiza Roxb. has long gained focus among tribal people of Java, for its medicinal properties mainly against gynaecological challenges. The present study aims to identify the most potent phytocompound present in the extract and determine primary mode of action accountable for cytotoxic activity of Curcuma zanthorrhiza rhizome extract against HPV16-positive SiHa cervical cancer cells.

Insights into the transformation of VO motif to VO, VO and VO motifs and their interconversion along with a detailed mechanistic study of their anti-cancer activity in SiHa cervical cancer cells.

The basic criteria for the formation of complexes with VO, VO and VO motifs from the VO motif and their interconversion were explored utilizing two multidentate O,N-donor hydrazone ligands namely, E-2-Hydroxy-N'-(4-oxopentan-2-ylidine)benzohydrazide (HL) and E-2-Hydroxy-N'-(4-oxo-4-phenylbutan-2-ylidine)benzohydrazide (HL), derived from the condensation of 2-hydroxybenzoylhydrazide with acetylacetone and benzoylacetone respectively. Under aerobic condition, the possibility of forming complexes with different motifs in different solvents with varying pH was examined theoretically by computational methods with results that were verified experimentally. This study reveals that under aerobic condition, complexes with VO (1,2) and VO (3, 4) motifs were formed in protic CHOH and neutral CHCl solvent respectively while the formation of complexes (5-14) with VO motif required protic CHOH solvent and higher pH (≥ 7).

A family of amphiphilic dioxidovanadium(V) hydrazone complexes as potent carbonic anhydrase inhibitors along with anti-diabetic and cytotoxic activities.

A family of dioxidovanadium(V) complexes (1-4) of the type [Na(HO)][VO(HL)] (x = 4, 4.5 and 7) where HL represents the dianionic form of 2-hydroxybenzoylhydrazone of 2-hydroxyacetophenone (HL, complex 1), 2-hydroxy-5-methylacetophenone (HL, complex 2), 2-hydroxy-5-methoxyacetophenone (HL, complex 3) and 2-hydroxy-5-chloroacetophenone (HL, complex 4), have been synthesized and characterized by analytical and spectral methods. These complexes exhibited the potential abilities to suppress the erythrocytes carbonic anhydrase enzymatic activity in type 1 and type 2 diabetic patients (in vitro), promising antidiabetic activity against T2 diabetic mice (in vivo).

Lignan enriched fraction (LRF) of Phyllanthus amarus promotes apoptotic cell death in human cervical cancer cells in vitro.

Phyllanthus amarus is widely grown in this sub-continent and used traditionally to treat many common ailments. In the present study, lignan rich fraction of P. amarus extract was used on cervical cancer cell lines (HeLa, SiHa and C33A) to study it's mechanism of cell death induction.

Structure-activity relationship on DNA binding and anticancer activities of a family of mixed-ligand oxidovanadium(V) hydrazone complexes.

The title family of mixed-ligand oxidovanadium(V) hydrazone complexes are [VO(HL)(hq)] (1) and [VO(HL)(hq)] (2), where (HL) and (HL) are the dinegative form of 2-hydroxybenzoylhydrazone of acetylacetone (HL) and benzoylacetone (HL), respectively, and hq is the mononegative form of 8-hydroxyquinoline (Hhq). Complexes were used to determine their binding constant with CT DNA using various spectroscopic techniques namely, electronic absorption, fluorescence and circular dichroism spectroscopy. The binding constant values suggest the intercalative mode of binding with the CT DNA and follow the order: 2 > 1.

Exploring the effect of substituent in the hydrazone ligand of a family of μ-oxidodivanadium(v) hydrazone complexes on structure, DNA binding and anticancer activity.

The reaction of 2-hydroxybenzoylhydrazine (Hbh) separately with equimolar amounts of [VO(aa)] and [VO(ba)] in CHCl afforded the complexes [VO(HL)] (1) and [VO(HL)] (2) respectively in good to excellent yield ((HL) and (HL) represent respectively the dianionic form of 2-hydroxybenzoylhydrazones of acetylacetone (HL) and benzoylacetone (HL) (general abbreviation HL)). From X-ray structure analysis, the V-O-V angle was found to be ∼115° and 180° in 1 and 2 respectively. Upon one-electron reduction selectively at one V centre at an appropriate potential, each of 1 and 2 generated mixed-valence [(HL)VO-(μ-O)-OV(HL)] species 1A and 2A respectively, which showed valence delocalization at room temperature and localization at 77 K, and the V-O-V bond angles were calculated to be 177.

Exploring the effect of hydroxylic and non-hydroxylic solvents on the reaction of [VO(β-diketonate)] with 2-aminobenzoylhydrazide in aerobic and anaerobic conditions.

Refluxing [VO(β-diketonate)], namely [VO(acetylacetonate)] and [VO(benzoylacetonate)], separately with an equivalent or excess amount of 2-aminobenzoylhydrazide (ah) in laboratory grade (LG) CHOH in aerobic conditions afforded non-oxidovanadium(iv) and oxidovanadium(v) complexes of the type [V(L)] (1), [VO(L)(OCH)] (3) and [V(L)] (2), and [VO(L)(OCH)] (4), respectively. (L) and (L) represent the dianionic forms of 2-aminobenzoylhydrazone of acetylacetone (HL) and benzoylacetone (HL), respectively, (general abbreviation, HL), which was formed by the in situ condensation of ah with the respective coordinated [β-diketonate] in medium-to-good yield. The yield of different resulting products was dependent upon the ratio of ah to [VO(β-diketonate)].