Construction and utilization of carotenoid reporter systems: identification of chromosomal integration sites that support suitable expression of biosynthetic genes and pathways.

In order to metabolically engineer microorganisms to produce compounds of interest, it is often desirable to integrate foreign genes into the chromosome of the host. However, the consequences of these genetic alterations are not always predictable. The use of a reporter system can often assist in determining chromosomal locations for optimal expression of foreign biosynthetic genes.

Modification of the BAX Salmonella test kit to include a hot start functionality (modification of AOAC Official Method 2003.09).

In 2010, the BAX System PCR assay for Salmonella was modified to include a hot start functionality designed to keep the reaction enzyme inactive until PCR begins. To validate the assay's Official Methods of Analysis status to include this procedure modification, an evaluation was conducted on four food types that were simultaneously analyzed with the BAX System and either the U.S.

Use of transposon promoter-probe vectors in the metabolic engineering of the obligate methanotroph Methylomonas sp. strain 16a for enhanced C40 carotenoid synthesis.

The recent expansion of genetic and genomic tools for metabolic engineering has accelerated the development of microorganisms for the industrial production of desired compounds. We have used transposable elements to identify chromosomal locations in the obligate methanotroph Methylomonas sp. strain 16a that support high-level expression of genes involved in the synthesis of the C(40) carotenoids canthaxanthin and astaxanthin.