Publications by authors named "Deana Colburg"

Strategies to enhance the anti-tumor immune response of stereotactic ablative radiotherapy (SABR) at primary tumors and abscopal sites are under intensive investigation. Here we report a metabolizable binary supracluster (BSC) that combines gold nanoclusters as radiosensitizing adjuvants with small interfering RNA (siRNA) targeting the immunosuppressive mediator galectin-1 (Gal-1). BSC comprises reversibly crosslinked cationic gold nanoclusters and siRNA complexes in a polymer matrix that biodegrades over weeks, facilitating clearance (90.

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Article Synopsis
  • In vitro models studying autoimmunity often lack the complex immune environment and epithelial interactions necessary for accurate research, particularly regarding coeliac disease (CeD), which involves gluten triggering immune responses.
  • Researchers created air-liquid interface (ALI) duodenal organoids from biopsies, preserving both epithelial cells and local immune cells, allowing for a more realistic study of CeD.
  • The findings highlighted that gluten peptides led to significant epithelial damage in CeD organoids, mediated by immune responses including the action of IL-7, a critical factor in the disease's pathology, especially in active cases compared to those on gluten-free diets.
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Salivary gland homeostasis and regeneration after radiotherapy depend significantly on progenitor cells. However, the lineage of submandibular gland (SMG) progenitor cells remains less defined compared with other normal organs. Here, using a mouse strain expressing regulated CreERT2 recombinase from the endogenous Tert locus, we identify a distinct telomerase-expressing (Tert) cell population located in the ductal region of the adult SMG.

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Tumor-associated macrophages are transcriptionally heterogeneous, but the spatial distribution and cell interactions that shape macrophage tissue roles remain poorly characterized. Here, we spatially resolve five distinct human macrophage populations in normal and malignant human breast and colon tissue and reveal their cellular associations. This spatial map reveals that distinct macrophage populations reside in spatially segregated micro-environmental niches with conserved cellular compositions that are repeated across healthy and diseased tissue.

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Tumor-associated macrophages (TAMs) display heterogeneous phenotypes. Yet the exact tissue cues that shape macrophage functional diversity are incompletely understood. Here we discriminate, spatially resolve and reveal the function of five distinct macrophage niches within malignant and benign breast and colon tissue.

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  • The study examines tenosynovial giant cell tumors (TGCT) and the role of bystander macrophages that respond to overproduced CSF1 from neoplastic cells with a chromosomal translocation of the CSF1 gene.
  • Using single-cell RNA sequencing (scRNA-seq) on 18,788 cells from TGCT and giant cell tumor of bone samples, researchers found two neoplastic cell populations similar to normal synoviocytes and identified GFPT2 as a key marker.
  • The neoplastic cells lack CSF1R, which suggests they may not respond to current treatments, but high GFPT2 expression indicates activation of signaling pathways that could be targeted for future therapies.
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Pancreatic cancer is one of the deadliest cancers, against which current immunotherapy strategies are not effective. Herein, we analyzed the immune cell composition of the tumor microenvironment of pancreatic cancer samples in The Cancer Genome Atlas and found that the presence of intratumoral NK cells correlates with survival. Subsequent analysis also indicated that NK cell exclusion from the microenvironment is found in a high percentage of clinical pancreatic cancers and in preclinical models of pancreatic cancer.

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During the SARS-CoV-2 pandemic, novel and traditional vaccine strategies have been deployed globally. We investigated whether antibodies stimulated by mRNA vaccination (BNT162b2), including third-dose boosting, differ from those generated by infection or adenoviral (ChAdOx1-S and Gam-COVID-Vac) or inactivated viral (BBIBP-CorV) vaccines. We analyzed human lymph nodes after infection or mRNA vaccination for correlates of serological differences.

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