Developmental polychlorinated biphenyl exposure influences adult zebra finch reproductive behaviour.

Polychlorinated biphenyls (PCBs) are worldwide chemical pollutants that have been linked to disrupted reproduction and altered sexual behaviour in many organisms. However, the effect of developmental PCB-exposure on adult passerine reproductive behaviour remains unknown. A commercial PCB mixture (Aroclor 1242) or an estrogenic congener (PCB 52) were administered in sublethal amounts to nestling zebra finches (Taeniopygia guttata) in the laboratory to identify effects of developmental PCB-exposure on adult zebra finch reproductive parameters.

Concerted and mosaic evolution of functional modules in songbird brains.

Vertebrate brains differ in overall size, composition and functional capacities, but the evolutionary processes linking these traits are unclear. Two leading models offer opposing views: the concerted model ascribes major dimensions of covariation in brain structures to developmental events, whereas the mosaic model relates divergent structures to functional capabilities. The models are often cast as incompatible, but they must be unified to explain how adaptive changes in brain structure arise from pre-existing architectures and developmental mechanisms.

Juvenile zebra finches learn the underlying structural regularities of their fathers' song.

Natural behaviors, such as foraging, tool use, social interaction, birdsong, and language, exhibit branching sequential structure. Such structure should be learnable if it can be inferred from the statistics of early experience. We report that juvenile zebra finches learn such sequential structure in song.

The effect of polychlorinated biphenyls on the song of two passerine species.

Polychlorinated biphenyls (PCBs) are synthetic chemical pollutants with demonstrated detrimental toxic and developmental effects on humans and wildlife. Laboratory studies suggest that PCBs influence behavior due to their effects on endocrine and neurological systems, yet little is known about the behavioral consequences of sublethal PCB exposure in the field. Additionally, specific PCB congener data (in contrast to total PCB load) is necessary to understand the possible effects of PCBs in living organisms since number and position of chlorine substitution in a PCB molecule dictates the toxicity and chemical fate of individual PCB congeners.

Motor pathway convergence predicts syllable repertoire size in oscine birds.

Behavioral specializations are frequently associated with expansions of the brain regions controlling them. This principle of proper mass spans sensory, motor, and cognitive abilities and has been observed in a wide variety of vertebrate species. Yet, it is unknown if this concept extrapolates to entire neural pathways or how selection on a behavioral capacity might otherwise shape circuit structure.

Captivity reduces hippocampal volume but not survival of new cells in a food-storing bird.

In many naturalistic studies of the hippocampus wild animals are held in captivity. To test if captivity itself affects hippocampal integrity, adult black-capped chickadees (Poecile atricapilla) were caught in the fall, injected with bromodeoxyuridine to mark neurogenesis, and alternately released to the wild or held in captivity. The wild birds were recaptured after 4-6 weeks and perfused simultaneously with their captive counterparts.

Development of a brain nucleus involved in song production in zebra finches (Taeniopygia guttata) is disrupted by Aroclor 1248.

We studied whether polychlorinated biphenyls (PCBs) may alter the development of song control brain nuclei in zebra finch (Taeniopygia guttata) offspring of pulse-exposed hens. We orally administered 40 microg of Aroclor 1248 to adult female finches before egg laying. When the progeny were 50 d old, we measured the volumes of the song control nuclei robustus arcopallialis (RA) and higher vocal center (HVC) using light microscopy.

Immediate early gene (ZENK) responses to song in juvenile female and male zebra finches: effects of rearing environment.

Accurate song perception is likely to be as important for female songbirds as it is for male songbirds. Male zebra finches (Taeniopygia guttata) show differential ZENK expression to conspecific and heterospecific songs by day 30 posthatch in auditory perceptual brain regions such as the caudomedial nidopallium (NCM) and the caudomedial mesopallium (CMM). The current study examined ZENK expression in response to songs of different qualities at day 45 posthatch in both sexes.

Failure to detect seasonal changes in the song system nuclei of the black-capped chickadee (Poecile atricapillus).

Most temperate songbird species sing seasonally, and the brain areas involved in producing song (the song system) vary in size alongside the changes in behavior. Black-capped chickadees (Poecile atricapillus) also sing seasonally, and we find that there are changes in the stereotypy and the length of the fee-bee song from the nonbreeding to the breeding season. Yet despite these changes, we fail to find any evidence of seasonal changes in the song system.

Adult female and male zebra finches show distinct patterns of spine deficits in an auditory area and in the song system when reared without exposure to normal adult song.

Male songbirds typically require exposure to normal adult conspecific song during development in order to learn a normal song of their own. Females require exposure to conspecific song during development in order to select high-quality, learned song over the incomplete song produced by males reared in isolation. Altering males' opportunity for song learning during development affects the neuroanatomy of brain regions involved in song production (the song system), but in females the neural effects of song learning are unknown.

Reproductive impairment in zebra finches (Taeniopygia guttata).

The effects of polychlorinated biphenyls (PCBs) as compounds that may disrupt endocrine activity and, consequently, alter reproductive performance were investigated in altricial zebra finches (Taeniopygia guttata). The breeding performance and breeding cycle of zebra finches differed significantly between nonexposed birds and those experimentally pulse-exposed to Aroclor 1248, a PCB compound (40 microg/bird). Aroclor-exposed birds showed significantly increased numbers of clutches laid, nests constructed per pair, incubation time per pair, and percentage of hatchling mortality compared to controls.

Cannabinoid inhibition improves memory in food-storing birds, but with a cost.

Food-storing birds demonstrate remarkable memory ability in recalling the locations of thousands of hidden food caches. Although this behaviour requires the hippocampus, its synaptic mechanisms are not understood. Here we show the effects of cannabinoid receptor (CB1-R) blockade on spatial memory in food-storing black-capped chickadees (Poecile atricapilla).

Where is the bird?

Patterns of song perception, learning, and expression differ across species, sexes, and individuals. We can understand the neurobiology of song better by paying attention to these differences. I focus selectively on a few of the studies done in my lab over in recent years to illustrate this.

Neural constraints on the complexity of avian song.

Why do birds sing? In many species, because the song attracts or retains a mate. Why do females pay attention? This paper reviews evidence that females may do so because male song can be an honest indicator of attributes of a male's brain that could contribute to his fitness or that of his young. Male songbirds learn and produce their songs using a set of brain regions collectively known as the song system.

Long-term memory for spatial locations in a food-storing bird (Poecile atricapilla) requires activation of NMDA receptors in the hippocampal formation during learning.

Food-storing birds use a form of long-term memory to recover their hidden food caches that depends on the hippocampal formation (HF). The authors assessed whether food-storing birds' long-term memory for spatial locations requires N-methyl-D-aspartate receptor (NMDA-R)-dependent synaptic plasticity. Black-capped chickadees (Poecile atricapilla) were given bilateral infusions of the NMDA-R antagonist AP5 into the hippocampus, and their memory on a spatial reference memory task was assessed.

Reversible inactivation of the hippocampal formation in food-storing black-capped chickadees (Poecile atricapillus).

The role of the hippocampal formation (HF) in memory processing was assessed in food-storing black-capped chickadees (Poecile atricapilla) by reversibly inactivating the HF during different memory tests. The memory tests required birds to remember a location based on spatial cues only, or based on a combination of both spatial and distinct visual cues. Inactivation of the HF impaired short-term spatial memory, but not visual-spatial memory.

Song and the song control pathway in the brain can develop independently of exposure to song in the sedge warbler.

Previous studies have shown that female sedge warblers choose to mate with males that have more complex songs, and sexual selection has driven the evolution of both song complexity and the size of the major song control area (HVc) in the brain. In songbirds, learning from conspecifics plays a major role in song development and this study investigates the effects of isolation and exposure to song on song structure and the underlying song control system. Sibling pairs of hand-reared nestling sedge warblers were reared to sexual maturity under two conditions.

Septum volume and food-storing behavior are related in parids.

The hippocampal formation (HF) of food-storing birds is larger than non-storing species, and the size of the HF in food-storing Black-Capped Chickadees (Poecile atricapillus) varies seasonally. We examined whether the volume of the septum, a medial forebrain structure that shares reciprocal connections with the HF, demonstrates the same species and seasonal variation as has been shown in the HF. We compared septum volume in three parid species; non-storing Blue Tits (Parus caeruleus) and Great Tits (Parus major), and food-storing Black-Capped Chickadees.

The distribution of substance P and neuropeptide Y in four songbird species: a comparison of food-storing and non-storing birds.

The distributions of the neuropeptides substance P (SP) and neuropeptide Y (NPY) were investigated in four songbird species that differ in their food-storing behavior. The food-storing black-capped chickadee (Parus atricapillus) was compared to the non-storing blue tit (Parus caeruleus) and great tit (Parus major) within the avian family Paridae, as well as to the non-storing dark-eyed junco (Junco hyemalis). All four species showed a similar distribution of SP throughout the brain with the exception of two areas, the hippocampal complex (including hippocampus (Hp) and parahippocampus (APH)) and the Wulst (including the hyperstriatum accessorium (HA)).

Variation in the volume of zebra finch song control nuclei is heritable: developmental and evolutionary implications.

In many songbird species, females prefer males that sing a larger repertoire of syllables. Males with more elaborate songs have a larger high vocal centre (HVC) nucleus, the highest structure in the song production pathway. HVC size is thus a potential target of sexual selection.

Expression of immediate early genes in the hippocampal formation of the black-capped chickadee (Poecile atricapillus) during a food-hoarding task.

Black-capped chickadees store food in many different locations in their home range and are able to accurately remember these locations. We measured the number of cells immunopositive for three different Immediate Early Gene products (Fra-1, c-Fos and ZENK) to map neuronal activity in the chickadee Hippocampal Formation (HF) during food storing and retrieval. Fra-1-like immunoreactivity is downregulated in the dorsal HF of both storing and retrieving chickadees compared to controls.

Seasonal changes in neuron numbers in the hippocampal formation of a food-hoarding bird: the black-capped chickadee.

The volume of the hippocampal formation (HF) in black-capped chickadees (Poecile atricapillus) varies across the seasons, in parallel with the seasonal cycle in food hoarding. In this study, we estimate cell density and total cell number in the HF across seasons in both juveniles and adults. We find that the seasonal variation in volume is due to an increase in the number of small and large cells (principally neurons) in the fall.

Greater song complexity is associated with augmented song system anatomy in zebra finches.

We revisited the relationship between brain anatomy and song behavior in zebra finches. Consistent with previous studies in other songbirds, we find that differences in volume of the telencephalic song control nucleus HVc is predictive of differences in repertoire size and phrase duration in zebra finches. We extend the study of brain and behavior correlations in song birds by showing that repertoire size in zebra finches can be predicted by variance in several brain nuclei, providing the first demonstration that volumetric differences across multiple components of a neural network are predictive of song behavior.

Song, sexual selection, and a song control nucleus (HVc) in the brains of European sedge warblers.

Female sedge warblers select males that have more complex songs as mates. This study tests two predictions concerning HVc, a telencephalic nucleus that is essential for song learning and production: first, that males with more complex songs will have a larger HVc, and second that males who pair successfully will have a larger HVc than unpaired males. Data on song composition and pairing status were collected from wild sedge warblers breeding in Hungary.