Two-Dimensional Culture Systems to Enable Mechanics-Based Assays for Stem Cell-Derived Cardiomyocytes.

Well-controlled 2D cell culture systems advance basic investigations in cell biology and provide innovative platforms for drug development, toxicity testing, and diagnostic assays. These cell culture systems have become more advanced in order to provide and to quantify the appropriate biomechanical and biochemical cues that mimic the milieu of conditions present . Here we present an innovative 2D cell culture system to investigate human stem cell-derived cardiomyocytes, the muscle cells of the heart responsible for pumping blood throughout the body.

Users and "teasers": failure to follow through with initial mental health service inquiries in a child and family treatment center.

This article examines the experience of families who contacted a child and family community mental health treatment center, but then did not follow through to receive even one session of diagnosis or treatment at the agency. The paper reports the base rate for this behavior in a large (N = 2,358) sample of cases who either only made inquiry or who received treatment at the agency. The inquiry group, or "teasers," tended to have children who were older than client groups, to have relatively more behavioral and fewer personality problems, to have demonstrated problems earlier than client groups, and to have parents who were slightly older.