[Chemical stability of Salvia miltirrhiza hairy root].

Objective: To study on the chemical stability of Salvia miltirrhiza hairy root.

Method: The rolA gene was detected by PCR in DNA and the chemical contituent variances were detected by HPLC.

Result: The rolA gene was found in all the 10 batches of the culfured hairy root.

[Functional genomics studies of Salvia miltiorrhiza II--gene expression profiling of different stage of hairy root].

Objective: Studying the gene expression profiling of different stage hairy root of Salvia miltiorrhiza, in order to find functional genes.

Method: The contents of second metabolites were determined by HPLC and gene expression profiling was detected by cDNA microarray. cDNA labeled with a fluorescent dye (Cy5 and Cy3-dCTP) was produced by Eberwine's linear RNA amplification method and subsequent enzymatic reaction.

[Functional genomics studies of Salvia miltiorrhiza I. Establish cDNA microarray of S. miltiorrhiza].

Objective: Establishing cDNA microarray, in order to study functional genomics of Salvia miltiorrhiza.

Method: Total RNA samples were prepared from S. miltiorrhiza roots using a modified CTAB method.

[Effects of methyl jasmonat on accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy root].

Objective: To study the effects of methyl jasmonate (MJ) on the accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy roots.

Method: After 18 day's suspension culture of S. miltiorrhiza hairy roots induced by Agrobacterium rhizogenes ATCC15834, the chemical elicitor--methyl jasmonat was added into 6-7V suspension cultures and at the same time, tanshinones contents (including cryptotanshinone and tanshinone II(A)) on the day 2, 6 and 9, after dealing with MJ, was quantified by HPLC.

Callus cultures of Annona squamosa for the production of annonaceous acetogenins.

Callus cultures of Annona squamosa were induced using different explants including petals, seed contents (megagametophyte and embryo) and fruits (mesocarp). Growth of the calli induced from the explants was found to be influenced by the type, concentration and ratio of auxin vs. cytokinin.