[Treatment of patients with rhegmatogenous retina detachment combined with non-secondary glaucoma].

Objective: To discuss the cause of disease, treatment and therapeutic effect in patients with rhegmatogenous retina detachment (RRD) combined with non-secondary glaucoma.

Methods: Clinical data of 28 patients with RRD combined with primary or congenital glaucoma were retrospectively analyzed.

Results: Twenty-five out of the 28 patients succeeded with one operation (89.

[The effects of caspase-3 in apoptosis of cultured retinal microvascular pericytes induced by advanced glycation end products].

Objective: One of the earliest changes observed in the development of diabetic retinopathy (DR) is the selective loss of pericytes and acellular capillaries. We tested the hypothesis that advanced glycation end products (AGE) might be involved in the disappearance of retinal pericytes by apoptosis and further investigated the activity and effect of caspase-3 at the same time.

Methods: Cultured bovine retinal microvascular pericytes (BRPs) were exposed to various concentrations of advanced glycation end products-bovine serum albumin (AGE, 0.

Advanced glycation end-products induce apoptosis involving the signaling pathways of oxidative stress in bovine retinal pericytes.

One of the histopathologic hallmarks of early diabetic retinopathy is the selective loss of pericytes. Evidences suggest that the pericyte loss in vivo is mediated by apoptosis. However, the underlying cause of pericyte apoptosis is not fully understood.

[Splicing site mutation of D19S418 in PRPF-31 gene and its phenotypic characters with autosomal dominant retinitis pigmentosa].

Objective: To evaluate the disease-causing gene and phenotypic characters of a large family with autosomal dominant retinitis pigmentosa (adRP).

Methods: Disease status and associated ocular abnormalities of eight patients and six unaffected members who represent different generations of this family were assessed by measurement of visual psychophysics, full-field and multifocal electrophysiology (ERG and mfERG) and funds fluorescent angiography (FFA). The DNA samples of nineteen patients and fifteen unaffected individuals in this family were examined by Genome scanning, linkage analysis and mutation detection to identify coding sequence changes.

[Corneal combined amniotic membrane transplantation for early severe alkali chemical injury of the eye].

Objective: To investigate the effect of lamella corneal transplantation combined amniotic membrane transplantation for the early severe alkali burns of eyes.

Methods: Twenty-three eyes with an over second-degree acute corneal alkali injury in 2 weeks were treated by lamella corneal transplantation combined amniotic membrane transplantation. After the operation the patients were treated with an intense and prolonged regimen of topical steroids and topical and systemic vitamin C.

[Cyclosporin A combined with dexamethasone in preventing and treating immune rejection after penetrating keratoplasty].

Objective: To evaluate the efficacy of topical 1% cyclosporin A (CsA), 0.1% dexamethasone or 1% CsA combined with 0.1% dexamethasone in preventing and treating immune rejection after penetrating keratoplasty (PKP).

[Modification of silicone oil removal via limbus of corneal with aphakia].

Objective: To introduce a simplified method to remove silicone oil via limbus of corneal after previous lensectomy-vitrectomy.

Methods: Fifty-one eyes received the modified procedures to eliminate silicone oil via one pars plana or limbus incision after previous lensectomy-vitrectomy. Local anesthesia was performed with 25G syringe needle through pars plana or corneal limbus infusion and super limbus incisions to eliminate silicone oil.

[Analysis of misdiagnostic causes of Coats disease].

Objective: To analyze the clinical features and misdiagnostic causes of Coats disease.

Methods: The clinical data were retrospectively analyzed in 69 eyes of 68 patients with Coats disease.

Results: Misdiagnostic histories to a varying degree occurred in 11 of the 68 cases with a misdiagnosis rate of 16.

[Transplantation of amniotic membrane and amniotic membrane combined with limbal autograft for patients with complicated pterygium].

Objective: To evaluate the curative effects of amniotic membrane transplantation and amniotic membrane combined with limbal autograft on patients of complicated pterygium.

Methods: Forty-two cases (48 eyes) of recurrent pterygium or pseudopterygium were operated on with human amniotic membrane transplantation (20 cases 22 eyes) and amniotic membrane combined with limbal autograft (22 cases 26 eyes). All cases were followed up for 6-12 months.

[Advanced glycation end products induce apoptosis and expression of apoptotic genes in cultured bovine retinal capillary pericytes].

Objective: The purpose of the present study was to examine whether advanced glycation end products (AGE) contribute to the development of apoptosis and expression of apoptotic genes in cultured bovine retinal capillary pericytes (BRPs), in order to investigate retinal microvascular pathologic process during early stage of diabetic retinopathy.

Methods: After a 4 day incubation with various concentrations of AGE (8, 32, 125, 500, 2,000 mg/L) which were prepared in vitro by incubating bovine serum albumin (BSA) with glucose, we studied the degree of apoptosis and the expressions of apoptotic genes (Bax and bcl-2) in BRPs by staining with the Annexin V-FITC and propidium iodide (PI), terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and immunohistochemistry.

Results: (1) After a 4 day incubation with various concentrations of AGE, BRPs showed typical changes of apoptosis, i.