Down-regulation of p16 and MGMT promotes the anti-proliferative and pro-apoptotic effects of 5-Aza-dC and radiation on cervical cancer cells.

Cervical cancer is one of the most common malignancies of the female reproductive system. Therefore, it is critical to investigate the molecular mechanisms involved in the development and progression of cervical cancer. In this study, we stimulated cervical cancer cells with 5-aza-2'-deoxycytidine (5-Aza-dC) and found that this treatment inhibited cell proliferation and induced apoptosis; additionally, methylation of p16 and O-6-methylguanine-DNA methyltransferase (MGMT) was reversed, although their expression was suppressed.

Three-step versus "see-and-treat" approach in women with high-grade squamous intraepithelial lesions in a low-resource country.

Objective: To investigate the frequency of cervical intraepithelial neoplasia (CIN) 2 or greater in women with high-grade squamous intraepithelial lesion (HSIL), and to evaluate whether colposcopically-directed biopsy is a necessary procedure for managing HSIL in a low-resource country.

Methods: A retrospective review was undertaken of women with HSIL on a Pap smear.

Results: Of 348 women who had undergone colposcopically-directed biopsies and loop electrosurgical excisional procedure (LEEP), 321 (92.

[Study on cervical intraepithelial neoplasia in relation to human papillomavirus and co-factors: a case-control study in China and Australia].

Objective: This study was to elucidate the role of human papillomavirus (HPV) types and cofactors in the development of cervical intraepithelial neoplasia (CIN).

Methods: Two hundred and twelve women with CIN and 427 women with normal cervical cytology (control group) were recruited from China and Australia. A questionnaire was administered to each participant to obtain the demographic and risk factor information.

[Combining high-risk human papillomavirus DNA test and cytological test to detect early cervical dysplasia].

Objective: To assess the value of combining high-risk human papillomavirus (HPV) DNA test and cytological test in detection of early cervical dysplasia.

Methods: During January 2003 to June 2004, a total of 5210 women were screened by combining high-risk HPV DNA test (hybrid capture II, HC-II) and cytological test (liquid-based ThinPrep cytology test), and the abnormal cytological or HPV DNA findings were further biopsied under the colposcope.

Results: The age of the patients was between 17 to 80, the average was 34 +/- 9.

[Pregnancy and delivery outcomes after loop electrosurgical excision procedure of cervical intraepithelial neoplasia].

Objective: To investigate the effects of loop electrosurgical excision procedure (LEEP) on pregnancy and delivery outcomes of patients with cervical epithelial neoplasia (CIN).

Method: The delivery data of 27 pregnant women who had LEEP from May 1995 to April 2002 were retrospectively collected and analysed.

Results: The mean age of the 27 women was 30.