[Nitrogen forms and its distribution character in immerged and water-level-fluctuating zone soils of the backwater reach from input river of Three Gorges Reservoir].

This study measures the contents of different nitrogen forms in the immerged soils and the soils of water-level-fluctuating zone of the backwater area from input river of Three Gorges Reservoir, and analyzes the relationship between nitrogen forms and environmental parameters. The results indicates that: (1) Content of TN in the surface immerged soils ranged from 436.0 to 921.

[Phosphorus forms and its distribution characteristics in sediments and soils of water-level-fluctuating zone of the backwater reach from input river of Three Gorges Reservoir].

The contents of different phosphorus forms in surface sediments and the soils of water-level-fluctuating zone of the backwater reach from input river of Three Gorges Reservoirwere measured, using thestandard measurement and test (SMT) procedure of phosphorus forms in freshwater sediments. Correlation coefficients between phosphorus forms and sample geochemical characteristics, such as organic matter and major components were analyzed. The results indicated that the TP content in surface sediments varied from 483.

Analysis of HLA alleles polymorphism in Chinese patients with primary biliary cirrhosis.

Background: A familial clustering of patients with primary biliary cirrhosis (PBC) and the presence of immunological abnormalities in family members suggest a genetic component involved in the pathogenesis of PBC. The aims of this study are to investigate the frequencies of human leukocyte antigen HLA-A, -B, and -DRB1 alleles in Chinese patients with PBC by polymerase chain reaction (PCR)-based techniques, and to assess the correlation of the above-mentioned HLA with some clinical and laboratory features.

Methods: Genotyping of HLA alleles were performed in 65 well-characterized PBC patients and 431 healthy controls with sequence-specific primers PCR amplification.

[Quantitation of antigen specific T lymphocytes in peripheral blood of patients with primary biliary cirrhosis using tetramer technology].

Aim: To quantitate antigen specific T lymphocytes in peripheral blood from patients with primary biliary cirrhosis(PBC) and study the role of antigen specific T lymphocytes in the development of PBC.

Methods: Using tetramers and CD8 monoclonal antibody staining, PDC-E2 159-167aa and PDC-E2 165-174aa specific CD8(+) T lymphocytes were determined respectively in the peptide-induced cytotoxic T cell lines prepared from peripheral blood mononuclear cells(PBMC) of 15 PBC patients. The frequencies of these two kinds of antigen specific T lymphocytes in HLA-A*0201 positive (A2(+)) PBC were compared with those in A2(-) PBC patients, patients with other A2(+) chronic liver diseases and healthy controls.

[Effect of silicon and latex urinary catheters: a comparative study].

Objective: To evaluate the effect of silicon and latex urinary catheters on urinary mucosa.

Methods: A randomized, controlled prospective clinical trial was carried out in 60 patients admitted in the Department of Neurosurgery, who required long-term use of an indwelling urinary catheter. The patients were divided into 2 equal groups with placement of silicon or latex urinary catheter, and routine laboratory urine test was performed on a regular basis and the patients feelings during catheter retention were recorded.

[Analysis of anti-microbial resistance of Escherichia coli that caused infection in urogenital system].

Objective: To study the anti-microbial resistance of Escherichia coli (E.coli) that caused infection in urogenital system.

Method: Anti-microbial susceptibility tests for 148 strains of E.

[Detection and analysis of SARS coronavirus-specific antibodies in sera from non-SARS children].

Objective: To examine the presence of severe acute respiratory syndrome (SARS) coronavirus-specific antibodies in the sera from non-SARS children.

Methods: Indirect immunofluorescent assay and double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) were used to detect the virus-specific antibodies in sera of 1,060 non-SARS children in Guangzhou.

Results: All the serum samples from the 1,060 non-SARS children were negative for both IgG and IgM antibodies against SARS coronavirus as determined by indirect immunofluorescent assay, with only two serum samples showing weak positivity for SARS coronavirus-specific antibodies identified by double-antigen sandwich ELISA.

[Assay of Acinetobacter SPP drug-resistance by Kirby-Bauer and Etest method].

Objective: To study the drug-resistance of Acinetobacter spp and observe whether antibiotic-beta- lactamase inhibitor complexing agent enhances the sensitivity of the drug-resistant bacteria to the antibiotics.

Methods: Susceptibility tests to the antibiotics were performed for 60 isolated strains of Acinetobacter spp with Kirby-Bauer (K-B) and Etest methods.

Results: The drug-resistance rate of the strains to cefoperazone, ampicillin, ticarcillin and piperacillin exceeded 55.