[An experimental study on biocompatibility of Bio-oss collagen with cultured bone marrow stromal cells].

Purpose: To study the biocompatibility of Bio-oss collagen with cultured bone marrow stromal cells.

Methods: The bone marrow stromal cells (BMSCs) of rhesus monkey were cultured with Bio-oss collagen in vitro. Cell attachment was observed with laser confocal microscope.

[The experimental study of creating a new rat scarring model by inserting absorbable gelatin sponge into rats' excisional wounds].

Objective: To explore the possibility of creating a rat new scar model by inserting gelatin sponge into rat excisional wounds.

Methods: Two full-thickness wounds were created in each of total 49 SD rats. In the Experimental group (n = 19), a regular incisional wound (1 cm) was created on the left side, and an excisional wound of 1.

[Experimental study on constructing small-caliber artery by tissue engineering approach].

Objective: To investigate the possibility of constructing small-caliber artery by means of tissue engineering.

Methods: Cell-PGA mixtures were made by separately seeding 1 x 10(7) smooth muscle cells and 5 x 10(6) endothelial cells isolated from neonate umbilicus onto PGA scaffold, the cell-PGA constructs were wrapped around a silicone tube before its implantation subcutaneously to nude mice and the mice were sacrificed in 2 and 6 weeks. The tissue engineered artery (TEA) were examined both grossly and immunohistochemically.

[Inhibiting scar formation in rat cutaneous wounds by blocking TGF-beta signaling].

Objective: TGF-beta plays a key role in wound scarring. This study explored the possibility of using gene therapy to inhibit wound scarring by blocking TGF-beta signaling.

Methods: In vitro, human normal dermal fibroblasts were infected with recombinant adenoviruses of truncated TGF-beta receptor II (tTGF-betaRII, 100 pfu/cell) and beta-galactosidase (beta-gal, 100 pfu/cell), and their effects on regulating TGF-beta1 gene expression were analyzed by Northern blot.