Publications by authors named "De-kun Chen"
Article Synopsis
- Cytokine storm is an excessive immune response where high levels of cytokines are released, often triggered by acute infections, affecting multiple cell types and varying across different diseases.
- This review discusses the biological mechanisms that lead to cytokine storms in infectious diseases and explores treatment strategies aimed at managing these responses from both the host’s and the pathogen’s viewpoints.
- Research suggests that targeted therapies can reduce tissue damage and help clear infections, with advancements in "multi-omics" profiling expected to improve treatment strategies for cytokine storms across various diseases.*
Article Synopsis
- Goat milk plays a crucial role in the growth of kids by offering beneficial bacteria, which are essential for their early development.
- This study examined the microbiota of colostrum and mature milk from 30 Saanen dairy goats, revealing that mature milk has greater microbial diversity than colostrum.
- Findings indicate that the composition of milk microbiota varies significantly during different lactation stages, highlighting the need for further research in dairy microbiology.
Triterpenoids with antiproliferative activities from the twigs and leaves of .
J Asian Nat Prod Res
August 2021
One new pentacyclic triterpenoid, urs-12,16-dien-3-one (), together with twelve known pentacyclic triterpenoids (), were isolated from the twigs and leaves of . Their structures were characterized by their 1D- and 2 D-NMR spectra analysis and mass spectra studies. Furthermore, all isolated compounds were tested the inhibitory effect on proliferation of six human cancer cell lines , including NCI-H441, NCI-H460, A549, SKOV3, hela, and caki-1 cells.
View Article and Find Full Text PDFArticle Synopsis
- Contagious caprine pleuropneumonia (CCPP) is a serious disease affecting goats, caused by a specific bacterium that leads to high rates of illness and death, resulting in major economic losses for goat farmers worldwide.
- The exact process by which CCPP causes damage in goats is not fully understood, but new research indicates that the accumulation of neutrophils, a type of immune cell, plays a key role in the lung damage seen in affected goats.
- The study found that the cytokine IL-17 is crucial in this process, as it drives neutrophils to the lungs and stimulates their production from lung cells, suggesting that targeting IL-17 could be an effective treatment strategy for CCPP.
Article Synopsis
- Interferon-gamma (IFN-γ) is a crucial component of the immune response with roles in antiviral actions and inflammation, and this study aimed to create a monoclonal antibody (mAb) against caprine IFN-γ using mice.
- The researchers produced recombinant caprine IFN-γ in E. coli and successfully developed a hybridoma (designated as 2C) that secretes mAb, which specifically recognizes the IFN-γ protein, as confirmed through various assays.
- The developed mAb 2C is effective in detecting IFN-γ in goats, showing promise for understanding its expression during contagious ecthyma infections.
Article Synopsis
- Viral infections are a major health issue globally, leading to severe illness and death, and provoke complex immune responses from the host to combat the virus.
- Interleukin (IL)-17 is a key cytokine involved in these responses, showing both protective and harmful effects during viral infections; it enhances immune responses but can also worsen the illness.
- Understanding the dual role of IL-17 is crucial for developing targeted immunotherapy, emphasizing that treatments need to be customized based on the specific virus and the infection context.
Article Synopsis
- The study focused on purifying caprine IL-17A (cIL-17A) to create a specific monoclonal antibody (mAb) for research on inflammatory and autoimmune diseases.
- Researchers cloned the cIL-17A coding sequence from goat blood cells and produced a recombinant fusion protein, which was used to immunize mice for antibody generation.
- The resulting monoclonal antibody H8 was effective in recognizing both the recombinant and natural forms of cIL-17A, suggesting its potential use for studying IL-17-related diseases.
Article Synopsis
- - Organisms have developed mechanisms to protect their DNA, but damage can occur, necessitating DNA repair pathways, especially for double-strand breaks in eukaryotes like mammals.
- - There are two main repair pathways: nonhomologous DNA end joining, which can lead to errors, and homologous recombination, which is more precise but less efficient.
- - The text discusses strategies to enhance the efficiency of homologous directed repair by manipulating the cell cycle, boosting key proteins for homologous recombination, and choosing suitable donor DNA.
The Role of Monocytes and Macrophages in Autoimmune Diseases: A Comprehensive Review.
Front Immunol
September 2020
Article Synopsis
- Monocytes and macrophages are crucial for the innate immune system, helping to manage inflammation and tissue repair while also influencing autoimmune diseases.
- These immune cells are often found in diseased tissues and may be linked to the progression of autoimmune disorders, but their exact role—whether they cause or worsen the disease—is not fully understood.
- Understanding the various types of monocytes and macrophages involved in different autoimmune conditions may pave the way for better treatment options in the future.
Article Synopsis
- The gut microbiota, consisting of over a trillion microbes, forms a mutually beneficial relationship with mammals, contributing to immune system development and pathogen defense.
- The microbiota utilizes several mechanisms to enhance the innate immune response, including the secretion of antimicrobial peptides and activation of the inflammasome.
- Key immune molecules like IL-22, IL-17, and IL-10 are produced in response to these interactions, showcasing the gut microbiota's role in protecting the host from infections.
Immunological abnormalities in patients with primary biliary cholangitis.
Clin Sci (Lond)
March 2019
Article Synopsis
- Primary biliary cholangitis (PBC) is an autoimmune liver disease primarily affecting women, marked by high levels of anti-mitochondrial antibodies and liver damage.
- Immune cells, especially CD4 T cells, play a vital role in the disease's progression by promoting inflammation and damaging bile ducts, which leads to serious conditions like liver fibrosis and cirrhosis.
- Research highlights the complexity of immune responses in PBC, suggesting that targeted immunotherapy could be an effective treatment, with different approaches needed based on the disease's stages.
Article Synopsis
- Contagious pustular dermatitis is a zoonotic disease linked to the orf virus, causing major economic issues during pandemic outbreaks.
- A study tested blood samples from 628 healthy goats in China and found the orf virus present in some of them.
- The virus from these asymptomatic goats was shown to be infectious, causing typical symptoms in uninfected goats, indicating that healthy-looking animals can still carry the virus and contribute to the disease's spread.
The giant panda (Ailuropoda melanoleuca) is an endangered species and indigenous to China. Interferon-gamma (IFN-γ) is the only member of type □ IFN and is vital for the regulation of host adapted immunity and inflammatory response. Little is known aboutthe FN-γ gene and its roles in giant panda.
View Article and Find Full Text PDFA survey of dairy goats for infection with Eimeria species of coccidia was conducted in the Shaanxi province, northwestern China between December and November 2010, including Saanen and Guanzhong breeds. A total of 584 fecal samples (250 and 334 from Saanen and Guanzhong dairy goats, respectively) in six farms were collected. Eimeria oocysts were seen in 568 (97.
View Article and Find Full Text PDFBackground: Toxoplasma gondii is an important zoonotic pathogen causing significant human and animal health problems. Infection in dairy goats not only results in significant reproductive losses, but also represents an important source of human infection due to consumption of infected meat and milk. In the present study we report for the first time seroprevalence of T.
View Article and Find Full Text PDF[Study on the cloning, in vitro expression and bioactivity of Ailuropoda melanoleuca Qinling subspecies interleukin-2].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
June 2009
Aim: To identify if any difference exisit between the population of Sichuan and Qinling giant panda. express IL-2 protein in BL21(DE3)with biological activity.
Methods: IL-2 was amplified by RT-PCR from adult Qinling giant panda peripheral blood lymphocyte which was induced by ConA, and cloned into prokaryotic expression vector of pET32a-IL-2.
[Expression of xenogentic epidermal growth factor receptor ectodomain and its antitumor effect].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
November 2008
Aim: To construct the EGFR protein vaccine and evaluate its antitumor effect.
Methods: The gene of chicken EGFR ectodomain was amplified by PCR, inserted into the pGEX-4T-2 vetor, and then exporessed in E.coli BL21 with glutathione S-transferase in a fusion protein form.