[Detection of the mutation in HBV polymerase gene by RFLP PCR method in hepatitis B patients treated with lamivudine].

Background: To investigate the mutation of HBV polymerase gene in chronic hepatitis B patients treated with lamivudine.

Methods: The restriction-fragment-length-polymorphism (RFLP) assay for HBV DNA sequence determination at the codon 528 and 552 in the HBV polymerase gene associated with lamivudine resistance in vitro. HBV DNA samples extracted from sera of 240 patients were subjected to PCR amplification with primer pairs F2/R2 (552), F3/R2 (528).

[Clinical manifestation, treatment, and outcome of severe acute respiratory syndrome: analysis of 108 cases in Beijing].

Objective: To investigate the clinical manifestations, treatment, and outcome of severe acute respiratory syndrome (SARS).

Methods: The clinical data of 108 SARS in-patients were analyzed.

Results: Among the 108 cases, 35 males (32.

[Heterogeneity of HIV strains isolated from different tissues of 3 AIDS patients].

Background: To reveal the characteristics of genotype and phenotype of HIV strains in blood and some tissues of AIDS patients.

Methods: The virus was isolated from peripheral blood mononuclear cell (PBMC),cerebrospinal fluid (CSF)and lymph nodes of 3 AIDS patients by coculture with PBMC stimulated by PHA for 72 hours from uninfected donor. The cytopathic effect of the HIV isolates was determined in cultured MT2 cell line.