Phthalate Metabolites Were Related to the Risk of High-Frequency Hearing Loss: A Cross-Sectional Study of National Health and Nutrition Examination Survey.

Background: Phthalate metabolites are pervasive in the environment and linked to various health issues. This study aimed to investigate the relationship between phthalate metabolites and hearing loss.

Methods: We conducted a cross-sectional study with 1713 participants based on the National Health and Nutrition Examination Survey 2015-2018.

Carboxyamidotriazole alleviates muscle atrophy in tumor-bearing mice by inhibiting NF-κB and activating SIRT1.

Cancer cachexia is a complex disorder characterized by inflammatory responses, and it is associated with poor performance status and high mortality rate of cancer patients. Carboxyamidotriazole (CAI), a noncytotoxic chemotherapy agent, shows anti-inflammatory features in the treatment of many diseases. Here, we investigated the preventive and therapeutic effects of CAI on muscle loss that occurred in mice with advanced Lewis lung carcinoma (LLC).

Therapeutic Effect of Carboxyamidotriazole on Adjuvant Arthritis in Rats.

Objective: To study the effect of carboxyamidotriazole (CAI) on adjuvant arthritis (AA) in rats.

Methods: The rats were randomly divided into normal group,two vehicle groups (polyethylene glycol 400 control and normal sodium control group), CAI-treated groups (10, 20, and 40 mg/kg) and positive control dexamethasone group. Freund's completed adjuvant was used to induce AA in rats.

[Anti-inflammatory and analgesic potency of carboxyamidotriazole, a tumoristatic agent].

Objective: To explore the potential anti-inflammatory and analgesic activities of carboxyamidotriazole (CAI).

Methods: A variety of animal models, including the croton oil-induced ear edema, the cotton-induced granuloma, the rat adjuvant-induced arthritis, were used to evaluate anti-inflammatory effect of CAI. Vascular endothelial growth factor (VEGF)--or histamine-stimulated local vascular permeability in mouse modulated by CAI was also determined.

Carboxyamido-triazole inhibits proliferation of human breast cancer cells via G(2)/M cell cycle arrest and apoptosis.

Carboxyamido-triazole (CAI), a voltage-independent calcium channel inhibitor, has been shown to be able to induce growth inhibition and apoptosis in cancer cells. In the present study, we demonstrate that CAI significantly inhibits proliferation of cultured MCF-7 human breast cancer cells in a dose-dependent manner with an IC(50) of approximately 26 microM. Reduced proliferation of MCF-7 cells in the presence of CAI correlated with accumulation of cells in G(2)/M phase and induction of apoptosis.

[Activation of Ca(2+)-activated K+ channels by oxyphenamone in rabbit mesenteric vascular smooth muscle cells].

Aim: To study the effects of oxyphenamone (Oxy) on activation of Ca(2+)-activated K+ channels in rabbit mesenteric vascular smooth muscle cells.

Methods: To measure the effect of Oxy on the Ca(2+)-activated K+ channel (BK (Ca) channel) activity in rabbit mesenteric vascular smooth muscle cells by using whole cell patch clamp techniques.

Results: Oxy reversibly increase BK (Ca) channel activity in rabbit mesenteric artery smooth muscle cells.

[Effect of G protein in the dual regulation of opioid receptor agonist on the delayed rectified potassium channels].

Objective: To observe the role of G protein in the dual regulation of opioid receptor agonist on the delayed rectified potassium channels.

Methods: Using whole-cell patch-clamp techniques applied to NG108-15 cells, investigate the effect of opioid receptor agonist on the delayed rectified potassium channels by administration of Guanosine-5'-0'-2-thiociphosphate (GDP beta S), Pertusis Toxin (PTX), Tetroacetic acid nueleoside diphosphate kinase (NDPK) and Adenosine-3' 5' cyclic monophosphate cAMP in the pipette solution.

Results: (1) GDP beta S could block the changes induced by both high and low concentration of (D-Pen2.

[Dual regulation by delta opioid receptor agonists on the delayed rectified potassium channels in NG108-15 cells].

Objective: To investigate the dual effects by the delta opioid receptor agonists DPDPE on the delayed rectified potassium channels in NG108-15 cells.

Methods: A series of outward currents were evoked in NG108-15 cells by depolarizing voltage from -50 mV to +80 mV at holding potential of -90 mV. These currents were delayed rectified potassium currents.

[Binding characteristics of new synthesized opioid receptor ligands to cloned mu opioid receptors stably expressed in CHO cell].

Objective: To determine the affinity of new opioid receptor ligands to cloned mu opioid receptors stably expressed in CHO cell.

Methods: The binding characteristics of the opioid ligand [3H] diprenorphine (3H-dip) were studied by cellular biological techniques and radioligands binding in cloned mu opioid receptors stably expressed in CHO cells in saturation binding experiments, and were followed by competition binding experiments with a variety of new synthesized opioid receptor ligands.

Results: The Kd and Bmax of [3H] diprenorphine bound to mu receptors were 1.