Inflammation and cutaneous nervous system involvement in hypertrophic scarring.

This study aimed to use a mouse model of hypertrophic scarring by mechanical loading on the dorsum of mice to determine whether the nervous system of the skin and inflammation participates in hypertrophic scarring. Results of hematoxylin-eosin and immunohistochemical staining demonstrated that inflammation contributed to the formation of a hypertrophic scar and increased the nerve density in scar tissue.Western blot assay verified that interleukin-13 expression was increased in scar tissue.

[Changes in cytokines in lymph and T lymphocyte subsets in lymph node of burned rats].

Objective: To observe the effect of burn on cytokines in lymph and T lymphocyte subsets in lymph node of rats.

Methods: Eighteen Wistar rats were used in the experiment. One of the hind limbs of each rat was immersed in 70 °C hot water for 30 s to reproduce 4%TBSA deep partial-thickness scald model (burn group), while the other hind limb was immersed in 22 °C warm water for 30 s to simulate scald (sham injury group).

[Role of lymphatics in bacterial translocation from intestine in burn rats].

Objective: To investigate the role of lymphatics in bacterial translocation from intestine of rats with burn.

Methods: Escherichia coli (E. coli) labeled with chloromethylbenzamidodialkylcarbocyanine (CM-DIL) were prepared.

[Attach importance to research on lymph circulation system after burns].

A series of pathophysiological changes in lymph circulation system occur after severe burns. We try to elucidate the importance through summarizing our experiments on some of the changes in lymph circulation based on rat and goat lymphatic fistula model since 1998. The lymphatic contraction frequency decreased while the lymph flow speed increased during burn shock stage.

[The significance of TGF-beta expression in scar in adenosine receptor A(2A) knockout mice].

Objective: To discuss the mechanism of scar hypertrophy in adenosine receptor A(2A) (A(2A) R) knockout mice.

Methods: Animal models of hypertrophic scar were established in 12 A(2A) R knockout mice and 12 wild-type mice as control. The thickness and the size of transverse section of the hypertrophic scar were observed by H-E staining.

[Expression of tumor necrosis factor related apoptosis inducing ligand and its receptor in thymus during early post-burn stage in rat with severe burns].

Objective: To investigate the effect of tumor necrosis factor related apoptosis inducing ligand (TRAIL) and its receptor on apoptosis in thymus during early post-burn stage in rat with severe burns.

Methods: Fifty Wistar rats were randomly divided into sham scald group (SS, n = 10) and burn group (n = 40). The apoptosis in thymus in rats was detected with annexin V/FITC-PI double staining at 4, 12, 24, 48 post-burn hours (PBH).

Lymph invasion route of bacteria and endotoxin and CD4+/CD8+ T cells ratio changes of draining lymph node of burn infection wound.

The aim of this study was to determine the lymphatic invasion route of bacteria and endotoxin of burn wounds infected by Pseudomonas aeruginosa and moreover, the effect of P. aeruginosa infection of the burn wound on the draining lymph node and lymph fluid. Male Wistar rats were subjected to unilateral hind limb burn+wound infected by P.

Changes in cytokine levels and CD4+/CD8+ T cells ratio in draining lymph node of burn wound.

The aim of this study was to determine the changes of cytokine levels in draining lymph fluid and the changes of CD4+/CD8+ T-cells ratio in draining lymph node of burn wound. Male Wistar rats were subjected to unilateral hind limb burn (burn limbs group) and contralateral hind limb without burn (control limbs group). On hours 6, 24, and 72 after burn, rats were killed; lymph fluid in the efferent lymph trunk of the common iliac lymph nodes (CILN) were collected; and lymph fluid Interferon-gamma (IFN-gamma), interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-alpha) concentrations were measured by enzyme-linked immunosorbent assay.

[Experimental study on antitumor effects of Shen Qi Jin Kang (SQJK)].

Background & Objective: SHEN QI JIN KANG (SQJK) capsule is a complex preparation, consisting of effective components extracted from radix astragali, ginseng, curcuma, etc. It has been demonstrated to be able to decrease tumor volume, increase life quality and prolong survival time in clinic application. The study was to investigate the antitumor effects of SQJK capsule in vivo and in vitro, and further explore the possible mechanisms.

[A basic study and clinical trial of 585 nm pulsed dye laser in the treatment of congestive scars].

Objective: To study the biological effects of 585 nm pulsed dye laser (FLPDL) in the treatment of congestive scar.

Methods: By histological study, collagen VG staining and microvascular staining, we investigated the changes of collagen fibers and the density of microvessels in the congestive scars after FLPDL treatment.

Results: Histological and immunohistochemistry examinations showed that FLPDL achieved normal vascularity in the scar after over 3 times of treatment.

[Expression and significance of TRAIL receptors in fibroblasts of hypertrophic scar].

Objective: To detect the expression of TRAIL receptors in fibroblasts of hypertrophic scar in the proliferative stage and explore its significance.

Methods: 30 samples of hypertrophic scar were taken from 30 burn cases in the proliferative stage. 30 samples of normal skin were taken as the control.

[The changes in the tumor necrosis factor alpha, interleukin-6 and interleukin-8 levels in the lymph and of the dynamics of the lymphokines during shock stage of rats with major burns].

Objective: To study the dynamic changes in the lymphokines and the changes in tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), interleukin-8 (IL-8) levels in the lymph during shock stage of rats with major burns.

Methods: Forty-two male adult Wistar rats were randomly divided into burn resuscitation group (A, n = 18), burn non-resuscitation (B, n = 18) and the control (C, n = 6) groups. The TNF-alpha, IL-6 and IL-8 levels in the lymph were determined with radioimmunoassay at 6, 24, 48 postburn hours (PBH).

[Clinical analysis of severe burn patients with sepsis during shock stage].

Objective: To investigate the pathogenesis, management and prognosis of severe burn patients with sepsis during shock stage.

Methods: Fourty-four patients inflicted with over 60% TBSA burn injury and admitted to our hospital within 48 hours after injury during the past 8 years were enrolled in the study. The application of antibiotics in this group of patients was analyzed.