[Expression of CCR3 and CD34 in lung tissues and bone marrow of Guinea pig asthmatic models].

Aim: To explore the mechanism of asthma, by evaluating the changes of CCR3 and EOS expression in the lung tissues and bone marrow of Guinea pig asthmatic models at different times.

Methods: Guinea pigs (GPs) were sensitized and challenged by OVA to establish the asthmatic model (A, B, C, D, E) . The GPs in control (N) were sensitized and exposed to sterile saline.

[Cryobiological characteristics of placental cord blood preserved in bioarchive auto-preserved liquid nitrogen system].

The aim of this study was to investigate the cryobiological characteristics of placental cord blood (PCB) cryopereserved by using BioArchive auto-preserved liquid nitrogen system (BioArchive system). After Hespan depletion of red blood cells, 5 ml mixture of DMSO and 10% Dextran 40 were added into 20 ml of enriched leukocyte. 53 PCB units were cryopreserved as following protocol: pre-freeze rate 10 degrees C/min, start freeze temperature -3 degrees C, end freeze temperature -10 degrees C to -15 degrees C, post freeze rate 2 degrees C/min, and end temperature -50 degrees C.