[Molecular basis and clinical transfusion of a family with Bw subtype of ABO blood group system].

Objective: To study a family with Bw subtype of ABO blood group system, and to review safety issues in relation with clinical transfusion.

Methods: The molecular basis for the blood type was studied with serological assay, polymerase chain reaction-sequence specific primer (PCR-SSP) and DNA sequencing, TA clone and haplotype analysis in one blood donor whose ABO blood group were difficulty typed and her family. The bioinformatics analysis was carried out by biological analysis software to investigate the change of structure and function of enzymes influenced by the change amino acid.

[Mutational analysis for FUT1 gene in two cases with para-Bombay blood type].

Objective: To study two cases of rare para-Bombay blood types Bmh and Amh in order to determine clinical strategies of blood transfusion.

Methods: ABO blood type was determined with serological assays. The samples were also genotyped with polymerase chain reaction-sequence specific primer (PCR-SSP) for potential mutations in α-1,2-fucosyltransferase gene (FUT1).

[Application of microcolumn gel immunoassay in screening the platelet antibody].

The purpose of this study was to explore the clinical value of the platelet antibody screening and typing in platelets transfusion by using microcolumn gel immunoassay (MGIA). The platelets antigen-antibody reactions including the antibody screen and blood crossmatch were detected by MGIA. The results indicated that the detection of platelet antibody showed positive in 30 cases of aplastic anemia (AA), 11 cases of myelodysplastic syndrome (MDS), 24 out of 25 cases of leukemia and 1 out of cases of other diseases, while detection of platelet antibody showed negative in 20 normal volunteer donors.

5-HT(3) receptors in the central amygdala mediate the modulation of thymus function in rats.

The aim of the present study was to investigate the modulatory role of activated 5-HT(3) receptors in the central amygdala (CeA) on mitogen concanavalin A (ConA)-stimulated proliferative response of thymocytes in rats and the underlying neuroendocrine regulation circuits. 1-phenylbiguanide (PBG), a putative selective 5-HT(3) receptor agonist, was administered by intraperitoneal (i.p.

[Withdrawal symptoms of ethanol are ameliorated by thymopentin in mice].

Objective: To explore the effect of thymopentin (TP5) on the choice of ethanol and ameliorating withdrawal symptoms (anxiety) of ethanol in mice.

Methods: Mice were administered ethanol (v/v) in schedular fashion: 5% (1 week), 10% (1 week) and 15% (4 weeks), followed with the free choice between ethanol and water. Ethanol/(ethanol+water)x100% [E/(E+W)x100%] was measured as an index of ethanol selection.

[Expressions of c-fos in different brain regions after microinjection of 5-HT3 receptors agonist in hippocampus].

Aim: To explore the neuronal circuit and possible pathway related to the 5-HT3 receptors in hippocampus neuroimmunomodulation.

Methods: SABC immunocytochemical staining was used to detect the c-fos expression.

Results: A large number of FOS-positive neurons expression were found in the hippocampus and brain cortex at 1 hour after microinjection of 5-HT3 receptor agonist (I-PBG) in the hippocampus, but as time went on, the number of FOS positive-neurons gradually decrease.