Blockade of lysophosphatidic acid receptors LPAR1/3 ameliorates lung fibrosis induced by irradiation.

Lung fibrosis is a common and serious complication of radiation therapy for lung cancer, for which there are no efficient treatments. Emerging evidence indicates that lysophosphatidic acid (LPA) and its receptors (LPARs) are involved in the pathogenesis of fibrosis. Here, we reported that thoracic radiation with 16Gy in mice induced development of radiation lung fibrosis (RLF) accompanied by obvious increases in LPA release and LPAR1 and LPAR3 (LPAR1/3) transcripts.

[The construction and expression of eukaryotic expression plasmid of Legionella pneumophila immunogenic protein].

Objective: To construct the recombinant plasmid of Legionella pneumophila immunogenic protein gene (ip) and detect the immunoprotein expression in NIH3T3 cells.

Methods: The immunogenic protein gene was amplified from DNA of Legionella pneumophila by polymerase chain reaction (PCR), then inserted into pcDNA3. 1 (+) vector.

[In vitro expression of Lgeionella pneumophila pilE gene and its immunogenicity].

Objective: To construct a recombinant plasmid containing Lgeionella pneumophila pilE gene, detect its expression in NIH3T3 cells and evaluate its immunogenicity.

Methods: PilE gene (LP) was amplified from Legionella pneumophila DNA by PCR and inserted into pcDNA3.1(+) vector to construct the recombinant plasmid pcDNA3.

[The cloned ctxA gene of vibrio cholerae expresses in E. coli].

Objective: To construct the expression vector of Vibrio cholerae ctxA gene, and realize the Vibrio cholerae ctxA gene to express in E. coli, and lay a basis for future research on the immunogenicity and immunoadjuvant.

Methods: The ctxA gene, an cholera toxin subunit gene (ctxA) of Vibrio cholerae, was obtained by polymerase chain reaction (PCR) from DNA of Vibrio cholerae, and cloned into prokaryotic expressed vector pET32a(+) with thioredoxin (Trx) gene.