[Periodontal health survey and analysis of influencing factors in 916 patients with type 2 diabetes].

Purpose: To investigate the periodontal health status of type 2 diabetes patients in the Endocrinology Department of Shanghai Sixth People's Hospital, to explore relevant influencing factors of chronic periodontitis, so as to provide epidemiological basis for targeted prevention and treatment of periodontal diseases in patients with type 2 diabetes.

Methods: Periodontal examination and questionnaire survey of patients with type 2 diabetes who visited the Endocrinology Department of Shanghai Sixth People's Hospital from November 2018 to December 2018 were conducted. Criteria in "Basic Methods for Oral Health Survey" recommended by WHO was referred to check and score the relevant periodontal indicators, including probing depth, bleeding on probing, attachment loss, calculus index, plaque index, etc.

[Relationship between eruption status of mandibular third molars and thickness of the lingual bone].

Purpose: To estimate the relationship between the eruption status of the mandibular third molars and the thickness of the lingual bone.

Methods: Cone-beam CT (CBCT) data of 187 patients who underwent mandibular third molar extraction from Jan 2016 to Dec 2018 were selected. Lingual bone thickness at the levels of mid-root and root-apex of the third molars were measured using GALIEOS Viewer software, and the relationship between the eruption status of the mandibular third molars and the thickness of the lingual bone was estimated.

Occlusal force characteristics of masseteric muscles after intramuscular injection of botulinum toxin A(BTX - A)for treatment of temporomandibular disorder.

Our aim was to evaluate the occlusal force and therapeutic efficacy of the masseteric muscles after intramuscular injection of botulinum toxin A (BTX-A) for the treatment of patients with concurrent temporomandibular disorders (TMD) and bruxism. Thirty patients with TMD associated with bruxism were randomised into three groups (n=10 in each group), and treated by bilateral intramuscular injection of BTX-A into the masseter, placebo, or control. We used an occlusal force analysis system to collect several measures of occlusal force such as duration of biting and closing, the maximum occlusal force, and the distribution of occlusal force.

Induction of Osteogenic Differentiation of Human Adipose-Derived Stem Cells by a Novel Self-Supporting Graphene Hydrogel Film and the Possible Underlying Mechanism.

Graphene and its derivatives have received increasing attention from scientists in the field of biomedical sciences because of their unique physical properties, which are responsible for their interesting biological functions. With a range of extraordinary properties such as high surface area, high mechanical strength, and ease of functionalization, graphene is considered highly promising for application in bone tissue engineering. Here, we examined the effect of using a self-supporting graphene hydrogel (SGH) film to induce the osteogenic differentiation of human adipose-derived stem cells (hADSCs).

Maxillary sinus floor elevation with a tissue-engineered bone composite of deciduous tooth stem cells and calcium phosphate cement in goats.

The study aimed to assess the effect of maxillary sinus floor elevation with tissue-engineered bone constructed from deciduous tooth stem cells (DTSCs) and calcium phosphate cement (CPC). The stem cells from goat deciduous teeth (SGDs) were isolated and transfected by means of the adenovirus with an enhanced green fluorescent protein gene (AdEGFP). As many as 18 bilateral maxillary sinuses of nine goats were randomly allocated into three groups (n = 6/group): group A (SGDs-CPC compound), group B (CPC alone) and group C (autogenous bone obtained from an iliac crest).

[The influence of individual nursing care on extraction of teeth for old outpatients].

Purpose: To assess the impact of individual nursing care on aged patients who underwent tooth extraction in outpatient clinic.

Methods: One hundred aged patients who required tooth extraction were allocated into the experimental group (with individual nursing care before extraction) or the control group (blank control). The level of patients' anxiety was evaluated by HAMA scale before and after extraction.

[Preliminary study of akermanite's effect on stem cells from goat deciduous teeth in proliferation and osteogenic differentiation].

Purpose: To explore the characters of attachment, proliferation and osteogenic differentiation of stem cells from goat deciduous teeth (SGDs) cultured on akermanite ceramic.

Methods: SGDs were collected with enzyme digestion, and cultured. Under culture condition in growth medium, actin filament labeling and CCK-8 analysis were used to reveal attachment and proliferation.

The concentrations of IL-8 and IL-6 in gingival crevicular fluid during nickel-chromium alloy porcelain crown restoration.

We explored gum irritation and cytotoxicity caused by nickel-chromium (Ni-Cr) alloy porcelain by interleukin-8 (IL-8), interleukin-6 (IL-6) and gingival crevicular fluid (GCF) volumes at different time points peri-crown restoration. This prospective study was conducted in 60 young adults. The total amount and concentrations of IL-8 and IL-6 per site, GCF volumes, and blood neutrophil counts were performed prior to and at 1 week, 3 months, and 6 months after Ni-Cr alloy-porcelain crown restoration.

[Ectopic osteogenesis of SGDs in goat's muscle pouch].

Purpose: To investigate ectopic osteogenesis of composites SGDs with porous calcium phosphate cement(pCPC) in goat's muscle pouch.

Methods: SGDs were cultivated with modified tissue culture techniques, then were induced into osteoblasts in the third passage, the osteogenic-induced SGDs were combined with pCPC and transplanted into the goat left dorsal muscle pouch, the pCPC without cells was transplanted into the right dorsal muscle pouch as negative controls. The transplants were harvested at 2-,4-,6-,8- week and prepared for histological examination.

[Experimental study on stem cells from goat deciduous teeth compounded with porous calcium phosphate cement for ectopic osteogenesis].

Purpose: To observe the biocompatibility and ectopic bone-like tissue formation of stem cells from goat deciduous teeth (SGDs) with porous calcium phosphate cement (pCPC).

Methods: The expression of STRO-1 on SGDs was measured with flow cytometry (FCM); the 4th passage SGDs were cultured in induced-mineralization medium in vitro for 7 days. Combined with pCPC, the adhesion and growth of the compounds were observed with scanning electron microscopy (SEM); the ectopic bone-like tissue formation was observed 8 weeks after the compounds implanted subcutaneously into the nude mice.

[Isolation, cultivation and induced-mineralization of dental pulp cells from goat deciduous teeth in vitro].

Purpose: To isolate and cultivate dental cells from goat deciduous teeth ,and explore changes of its biological characters before and after induced-mineralization.

Methods: Pulp cells were cultivated with modified tissue block enzymolytic method, cell lineage in the second passage with SAB methods was checked out. Induced-mineralized cultivation was adopted in the fourth passage, some examinations were used to compare with normal cultivated cells: cell proliferative capality, mineralized ability test, cell morphology change, protein(OCN) expression level, related osteogenic genes(ALP,COL-I,OCN,OPN) expression.

[Culture and green fluorescent protein gene transfection of pulp cells from goat deciduous teeth in vitro].

Purpose: To isolate and culture pulp cells from goat deciduous teeth, and transfect green fluorescent protein gene.

Methods: Pulp cells from goat deciduous teeth were obtained by tissue culture. Cell growth curve was measured by counting the number of cells, HE and alkaline phophatase(AKP) stain, as well as immunhistochemical stain of vimentin and keratin were performed.

[The study of the monomer release from four kinds of temporary crown material by infrared spectrum].

Purpose: To study the quantity and characteristic of monomer release from four kinds of temporary crown material.

Methods: The experimental animal model was established by temporary prosthesis on dogs' teeth using four kinds of temporary crown materials.The quantity of residual monomer in temporary crown material was measured by infrared spectra at four different periods before and after wearing crowns.

[A study of osteogenic induction of dental pulp stem cells from deciduous teeth in vitro].

Unlabelled: URPOSE: To isolate and culture dental pulp stem cells from human deciduous teeth, and induce them to osteoblasts.

Methods: Dental pulp stem cells were separated from deciduous teeth with enzyme digestionìthen isolated and purified by limited dilution. The cells clone form rate was carried out, and cell growth curve was measured by counting the number of cells.

[Re-osseointegration of peri-implantitis bone defects: an experimental study in dogs].

Purpose: To investigate the treatment methods of experimentally induced peri-implantitis bone defects, and evaluate bone regeneration and re-osseointegration around implants histologically.

Methods: 30 Brinemark standard implants were placed in 5 Beagle dogs' mandibles to establish peri-implantitis bone defect model, then bone defects were randomly treated with: (1) debridement alone; (2) debridement plus guided bone regeneration using non-absorbable membrane; (3) debridement plus non-absorbable membrane associated with bone graft; (4) debridement plus non-absorbable membrane associated with biological activity glass. Peri-implant bone augmentation was observed and detected by radiography, fluorescence light micrography, un-decalcified bones ground section and direct measurement on operation.

[Detection and measurement of interleukin-8 in peri-implant crevicular fluid].

Objective: Gingival crevicular fluid (GCF) analysis of various inflammatory mediators has been investigated as a means of providing objective criteria of peri-implant tissue health. In this report, the crevicular fluid levels of interleukin-8 (IL-8), GCF volume and clinical parameters were studied.

Methods: GCF was sampled from 35 healthy and 35 inflamed sites of implantation patients.