Objective: To compare the quality of volatile oil of Acori Tatarinowii Rhizoma from Guangxi and Sichuan.
Methods: The volatile oil was extracted from Acori Tatarinowii Rhizoma by using steam distillation method, and analyzed by GC-MS. Peak area normalization method was used for calculating the relative percentage contents of chemical constituents, and hierarchical cluster analysis was used for classifying the 20 batches of samples by their relative contents of the main components that were methyleugenol, cis-methylisoeugenol, γ-asarone, β-asarone and α-asarone.
Objective: To observe the effects of volatile oil of Rhizoma Acori Tatarinowii (VOSCP) on morphology and cell viability in cultured neonate rat cardiac myocytes.
Methods: The cardiac myocytes were cultured by trypsin under cool treatment, and the cell purity was assayed with imminocytochemistry; Morphological changes were observed under phase contrast microscope after cardiac myocytes with VOSCP of different concentration for 24h in vitro, and the cell viability was examed by MTT assay.
Results: The purity of cultured neonate rat cardiac myocytes was higher than 95%; VOSCP of different concentration could depress pulse frequency and 100-160 mg/L VOSCP can obviously improve the viability of cardiac myocytes.