Heme oxygenase-2 suppress TNF-α and IL6 expression via TLR4/MyD88-dependent signaling pathway in mouse cerebral vascular endothelial cells.

Heme oxygenase (HO) represents an intrinsic antiinflammatory system based on its ability to inhibit expression of proinflammatory cytokines. The constitutive isoform heme oxygenase-2 (HO-2) has high expression and activity in cerebral microvascular endothelial cells (CMVEC). This study was undertaken to evaluate the role of HO-2 in regulation of TLR4/MyD88-dependent signaling and to study the effect of HO-2 on the expression and secretion of the proinflammatory cytokines tumor necrosis factor α (TNF-α) and Interleukin-6 (IL6) in CMVEC.

The immunosuppression mechanism of hypodermin A on complement.

Hypodermin A (HA), a serine protease secreted by first-instar larvae of Hypoderma lineatum (Diptera: Oestridae) is associated with inflammatory and the specific immune responses in cattle hosts. In the present study, the cDNA sequence of HA was synthesized, and found to have fifteen amino acids which differed from the sequence available in GenBank. We then examined the association between recombinant HA and guinea-pig complement component 3 (C3) through a co-immunoprecipitation assay.

[Genetic polymorphism of the IL8 gene and its associations with milk traits and SCS in Chinese Holstein].

The polymorphism of Interleukin-8 (IL8) gene were investigated for 610 Chinese Holstein cows of 30 bull families from a dairy farm in Shanghai using Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) technique with a mixed animal model to verify the effects of the polymorphisms on some milk productive performance, tested day milk yield, tested day fat percentage, tested day milk protein percentage, 305 d corrected milk yield, 305 d milk fat yield, 305 d milk protein yield, and somatic cell score (SCS). The aim was to explore the significant molecular marker in practical dairy production. Three genotypes were identified and the genotypic frequencies of KK, KA, and AA were 0.

[Molecular phylogeny of the gayal inferred from the analysis of cytochrome b gene entire sequences].

The gayal (Bos frontalis) is a very rare, semi-wild and semi-domestic bovine species. There still exist remarkable divergences on the gayal's origin and phylogenetic status. The cytochrome b (Cyt b) gene entire sequences (1,140 bp) of 11 gayals were sequenced and analyzed.

[Analysis of genetic co-adaptability of structural loci in Hu sheep].

Using the method of multiloci electrophoresis, genetic co-adaptability of Hu Sheep was analyzed according to allele frequencies in 14 structural loci. The results showed that no co-adaptability existed in the dominance-dominance model, while co-adaptability was observed in X-p-Cat combination locus of dominance-codominance model and in Po-CA, Po-Cat combination locus of codominance-codominance model. The findings demonstrated that co-adaptability occurred among neutral structural loci and played a key role in maintaining genetic situation, equilibrium or disequilibrium.

[Gene flow among sheep breeds of Mongolian group in China].

Gene flow was analyzed among four sheep breeds of Mongolian Group in China using structural gene loci and microsatellite markers.Results showed that genetic differentiation was 0.0164-0.