Publications by authors named "De-Fu Zhi"
Cationic lioposomes with folic acid as targeting ligand for gene delivery.
Bioorg Med Chem Lett
August 2016
In our previous Letter, we have carried out the synthesis of a novel DDCTMA cationic lipid which was formulated with DOPE for gene delivery. Herein, we used folic acid (FA) as targeting ligand and cholesterol (Chol) as helper lipid instead of DOPE for enhancing the stability of the liposomes. These liposomes were characterized by dynamic laser scattering (DLS), transmission electron microscopy (TEM) and agarose gel electrophoresis assays of pDNA binding affinity.
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- The study examined the effectiveness of two gene transfer reagents, Lipofectamine 2000 and DOTAP, in transfecting three different cell types: Hep-2, MCF-7, and SW-480.
- Results showed that Lipofectamine 2000 generally performed better across all cell lines, while DOTAP was particularly effective with Hep-2 cells but less so with MCF-7 and SW-480.
- Both reagents maintained high cell viability (over 85%) in MCF-7 cells, indicating their potential for safe application, although Lipofectamine 2000 demonstrated broader transfection efficiency overall.