Publications by De-Feng Tian

Publications by authors named "De-Feng Tian"

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Development of a new high-throughput screening model for human high density lipoprotein receptor (CLA-1) agonists.

Biomed Environ Sci

August 2005

Objective: To develop a new high-throughput screening model for human high-density lipoprotein (HDL) receptor (CD36 and LIMPII analogous-1, CLA-1) agonists using CLA-1-expressing insect cells.

Methods: With the total RNA of human hepatoma cells BEL-7402 as template, the complementary DNA (cDNA) of CLA-1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR). Bac-to-Bac baculovirus expression system was used to express CLA-1 in insect cells.

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