[The role of phospholipase D in cellular signaling].

Phospholipase D (PLD) hydrolyzes structural phospholipids of biological membrane to produce phosphatidic acid (PA) and a free-head group. Both of these compounds can participate in signal transduction. Since the activation of PLD involved in many cellular signaling cascades was identified in recent years, knowledge of and interest in PLD have grown greatly, and significant progress has been made toward understanding PLD.

[Transformation of Populus tomentosa with anti-PLD gene].

Antisense Phospholipase Dgamma( PLDgamma) gene was introduced into Populus tomentosa mediated by Agrobactrium tumefaciens. The young leaves of triploid populus were used as the material and the regeneration system of high frequency has been established. We have developed the traditional transgene method by Agrobactrium trmefaciens and obtained many transgenic plants of anti-PLDgamma gene.

[Analysis of salt resistance on the poplar transferred with salt tolerance gene].

Poplar(Pupulus x Xiao zhannica, cv. "balizhuang-yang") transferred with mtl-D gene was used as an experimental material. Through tissue culture, aquaculture and pot culture transgenic poplars are tested with variant Nacl salt gradient.