A novel APP splice variant-dependent marker system to precisely demarcate maturity in SH-SY5Y cell-derived neurons.

SH-SY5Y, a neuroblastoma cell line, can be converted into mature neuronal phenotypes, characterized by the expression of mature neuronal and neurotransmitter markers. However, the mature phenotypes described across multiple studies appear inconsistent. As this cell line expresses common neuronal markers after a simple induction, there is a high chance of misinterpreting its maturity.

Candida albicans Infection Model in Zebrafish (Danio rerio) for Screening Anticandidal Drugs.

Background: Candida albicans is an opportunistic fungal pathogen which causes systemic infections in human. In this study, C. albicans infection model was developed in zebrafish to understand the host-pathogen interactions for straightforward anticandidal drug screening.

Stress-immune responses and DNA protection function of thioredoxin domain containing 12 in zebrafish (Danio rerio).

Proteins with dithiol-disulfide oxidoreductase catalytic domain are well known for their capacity in the cellular redox homeostasis. In this study, we characterized the zebrafish thioredoxin domain containing 12 (Zftxndc12) gene, analyzed the transcriptional responses and studied the functional properties of its recombinant protein. Full-length cDNA of Zftxndc12 consists 519 bp coding region encoding 172 amino acids (AA) including the signal peptide.

Isolated from Rainbow Trout in Korea: Characterization, Anti- Activity and Host Pathogen Interaction in Zebrafish Disease Model.

Saprolegniasis is one of the most devastating oomycete diseases in freshwater fish which is caused by species in the genus including . In this study, we isolated the strain of from diseased rainbow trout in Korea. Morphological and molecular based identification confirmed that isolated oomycete belongs to the member of , supported by its typical features including cotton-like mycelium, zoospores and phylogenetic analysis with internal transcribed spacer region.

Preparation, Characterization, and Antimicrobial Properties of Chitosan-Silver Nanocomposites Films Against Fish Pathogenic Bacteria and Fungi.

Abstract: Development of nanostructured films using natural polymers and metals has become a considerable interest in various biomedical applications. Objective of the present study was to develop silver nano particles (AgNPs) embedded chitosan films with antimicrobial properties. Based on the Ag content, two types of chitosan silver nano films, named as CAgNfs-12 (12 mM) and CAgNfs-52 (52 mM) were prepared and characterized.

Chitosan silver nanocomposite (CAgNC) as an antifungal agent against Candida albicans.

Due to limited numbers of antifungal drugs and emergence of drug resistance have directed to develop nonconventional therapeutic agents against fungal pathogen Candida albicans. In this study, anticandidal activity of chitosan silver nanocomposite (CAgNC) was tested against C. albicans Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of CAgNC were determined as 25 and 100 μg/ml, respectively.

First report of Fusarium oxysporum species complex infection in zebrafish culturing system.

Fusarium oxysporum species complex (FOSC) is a highly diverse fungus. Recently, F. oxysporum infection was identified from zebrafish (Danio rerio) culturing system in Korea.