Publications by authors named "Dbc Lima"
Article Synopsis
- Testicular vitrification is a method used to preserve the genetic material of pre-pubertal animals, but there’s limited research on how to effectively warm vitrified testicular tissues.
- This study assessed how different warming temperatures (50, 55, and 60°C) affect the integrity and viability of vitrified testicular fragments from pre-pubertal cats through histological and mitochondrial activity evaluations.
- Results indicated that warming at 50°C maintained tubular integrity and cell junctions better than higher temperatures, with 60°C causing significant damage, making 50°C the optimal temperature for preserving testicular morphology and function.
Article Synopsis
- Systematic cryo-banking of black-footed ferret semen and testicular tissues can help preserve their genetic diversity and enhance assisted reproduction efforts for this endangered species.
- Research evaluated the viability and structural properties of vitried testicular tissues after warming and subsequent short in vitro culture, revealing that many aspects of tissue quality recovered to levels similar to fresh samples.
- Findings suggest that cryopreserved testicular tissues can support new reproductive strategies and conservation efforts for black-footed ferrets.
Understanding critical roles of warming and reanimation is critical to improve the survival of vitrified testicular tissue in domestic cats. The objective was to study structural and functional properties of testicular tissues from prepubertal domestic cats after standard vitrification followed by two warming protocols (directly at 37°C or with a 5-second pre-exposure to 50°C) and three reanimation time points (immediately, 24 h and 5 days post-warming). In Experiment 1, tissues were evaluated for histo-morphology and mitochondrial activity immediately or 24 h after warming protocols.
View Article and Find Full Text PDFProtocols for the cryopreservation of testicular tissue are not yet established. In cats, few studies have been conducted on testicular vitrification using different cryoprotectant associations (CPAs). Thus, the objective of this study was to compare the effect of different CPAs on the vitrification of testicular tissue from prepubertal cats in cryotubes.
View Article and Find Full Text PDFProstatomegaly is a common finding in older non-neutered dogs. This study compared the serum testosterone, sperm quality and characteristics of the prostatic fraction between healthy dogs and dogs with prostatomegaly. Blood samples of ten dogs (five dogs from each group) were taken for serum testosterone measurement.
View Article and Find Full Text PDFThe present study was conducted to identify the major proteome of the sperm-rich fraction and prostatic fraction of canine seminal plasma. Three semen samples from four healthy dogs were obtained by digital manipulation. The pre-sperm fraction, sperm-rich fraction and prostatic fraction were separated from each ejaculate.
View Article and Find Full Text PDFThe cryopreservation of testicular tissue is presented as the only alternative for the preservation of genetic material from prepubertal animals. However, this biotechnology is still being tested. The objective of this study was to evaluate the effect of different associations of cryoprotectants and the potential of cell proliferation after vitrification of testicular tissue of prepubertal cats.
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