Characterization of the cytochrome b(6)f complex from marine green alga, Bryopsis corticulans.

A pure, active cytochrome b(6)f was isolated from the chloroplasts of the marine green alga, Bryopsis corticulans. To investigate and characterize this cytochrome b(6)f complex, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), absorption spectra measurement and HPLC were employed. It was shown that this purified complex contained four large subunits with apparent molecular masses of 34.