Publications by authors named "Dayoung Yun"

Saccharomyces cerevisiae accumulates glycogen, a hyperbranched glucose polymer with multiple bio-functionalities. In this study, mutants of S. cerevisiae that accumulate excessive amounts of glycogen were developed through UV mutagenesis.

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Article Synopsis
  • CRISPR technology has changed genome editing but faces challenges with safe and efficient delivery methods for therapeutic uses.
  • The droplet cell pincher (DCP) microfluidic system offers a solution by enabling controlled and efficient delivery of CRISPR systems into cells, using mechanisms that create openings in cell membranes.
  • DCP has shown superior performance over traditional electroporation, achieving significantly higher success rates in genetic modifications like single and double knockouts and knock-ins, making it a promising tool for future CRISPR applications.
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The aim of this study is to develop a rapid and accurate method for simultaneous analysis of multi-residue pesticides and conduct pesticide monitoring in agricultural products produced by the production and distribution stage in Korea. The representative agricultural products were selected as brown rice, soybean, potato, mandarin, and green pepper and developed using gas chromatography with tandem mass (GC-MS/MS) for the analysis of 272 pesticide residues. The experimental samples were extracted by the QuEChERS-EN method and then cleaned up by using d-SPE, including MgSO and primary secondary amine (PSA) sorbents.

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Clustered regularly interspaced short palindromic repeats (CRISPR)- CRISPR-associated protein 9 (Cas9) genome editing technology is widely used for gene editing because it provides versatility in genetic manipulation. Several methods for regulating CRISPR activity already exist for accurate editing, but these require complex engineering. Thus, a simple and convenient regulatory system is required.

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Article Synopsis
  • Chlorothalonil is a widely used organochlorine fungicide that prevents fungal diseases in crops but can leave residues in agricultural products.
  • A new analytical method using a modified QuEChERS technique combined with gas chromatography-mass spectrometry (GC-MS/MS) was developed to effectively detect chlorothalonil residues in various foods in South Korea.
  • The method demonstrated high accuracy with excellent linearity and satisfactory detection limits, confirming chlorothalonil residues in multiple commodities including apples, watermelon, and cucumber, which can aid in food safety monitoring.
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Poly(2,6-dimethyl-1,4-phenylene oxide)s (PPOs)-based anion exchange membranes (AEMs) with four of the most widely investigated head groups were prepared. Through a combination of experimental and simulation approaches, the effects of the different types of head groups on the properties of the AEMs, including hydroxide conductivity, water content, physicochemical stability, and fuel cell device performance were fully explored. Unlike other studies, in which the conductivity was mostly investigated in liquid water, the conductivity of the PPO-based AEMs in 95% relative humidity (RH) conditions as well as in liquid water was investigated.

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