Impact of salinity stress on shifting microbial community and regulating NO and CO dynamics in alkaline wetlands.

Increasingly severe soil salinization in alkaline wetland due to elevated water evaporation under climate warming affected biogeochemical cycling processes, further threatening ecosystem imbalance and global greenhouse gas (GHG) budget. To reveal the underlying relationship between microbial dynamics, nitrous oxide (NO) and carbon dioxide (CO) characteristics under salinity stress in alkaline wetland, a 40-day microcosm experiment was conducted using soil collected from Zhalong wetland in northern China. The physiochemical properties, bacterial community, NO and CO emissions were observed in responses to different salinity gradients (0%, 0.

New insights into biofilm formation and microbial communities in hybrid constructed wetlands with functional substrates for treating contaminated surface water.

In this study, hybrid constructed wetlands (HCW) with functional substrates (vermiculite-tourmaline modified polyurethane) were constructed to investigate nitrogen removal efficiency and metabolic cooperation mechanisms for treating rural contaminated surface water with natural temperature fluctuations. The results show that within a natural temperature fluctuation range of 9-25 °C, the HCW achieved an average nitrate nitrogen removal efficiency of 98 % and a total nitrogen removal efficiency of 76 %, with effluent total nitrogen less than 5 mg/L. The rational secretion of extracellular polymeric substance and the analysis of microbial community structure revealed that functional substrate favors biofilm formation, increases the activity of Candidatus_Brocadia and Thauera, and enhances ammonia and nitrate reduction.

Enhanced nitrogen removal and microbial community of the mainstream deammonification treating fluctuating influent C/N wastewater by the novel functional carriers.

The plug-flow fixed bed reactors with zeolite/tourmaline-modified polyurethane carriers (PFBR) and polyurethane carriers (PFBR) were operated to assess the fluctuating influent C/N impact on the system performance and the carrier effect on the enhancing the system operation. Result suggested that fluctuations in influent C/N and variations in operational temperature reduced the removal performance and system stability within PFBR. The negative impact of C/N fluctuation could be effectively mitigated by effluent reflux.

Enzymatic bioremediation of polycyclic aromatic hydrocarbons (PAHs) in contaminated soil: a study on the recombinant laccase TVL.

Polycyclic aromatic hydrocarbons (PAHs) are pervasive and persistent pollutants in contaminated soil, posing a severe health and environmental threat. Enzymatic bioremediation presents a viable solution for the remediation of PAH-contaminated soil. In this study, a recombinant laccase with the encoding gene originating from and recombinantly expressed in designated as TVL was discovered to possess strong PAH reduction capabilities.

Bioremediation of PAHs-contaminated site in a full-scale biopiling system with immobilized enzymes: Removal efficiency and microbial communities.

Bioremediation of PAHs-contaminated soil by immobilized enzymes is a promising technology. Nevertheless, the practical implementation of highly efficient enzymatic remediation remains confined to laboratory settings, with limited experience in full-scale applications. In this study, the extracellular enzymes from white rot fungi are fully applied to treat sites contaminated with PAHs by combining a new hydrogel microenvironment and a biopiling system.

Removal of benzo[a]pyrene from the soil by adsorption coupled with degradation on saponin-modified bentonite immobilized crude enzymes.

Bentonite is a non-metallic mineral with montmorillonite as the main component. It is an environmentally friendly mineral material with large reserves, wide distribution, and low price. Bentonite can be easily modified organically using the surfactant saponin to obtain saponin-modified bentonite (Sap-BT).

Enhance PD/A biofilm formation via a novel biochar/tourmaline modified-biocarriers to treat low-strength contaminated surface water: Initial adhesion and high-substrate microenvironment.

In this work, a novel polyurethane carrier modified with biochar and tourmaline/zeolite powder at ratio of 1:1 and 1:2 was developed to promote the formation of biofilms and the synergy of overall bacterial activity for Partial Denitrification/Anammox to treat low-nitrogen contaminated surface water. Based on the batch experiment, the modified biocarrier, BTP2 (biochar: tourmaline = 2: 1), exhibited the highest total nitrogen removal efficiency (83.63%) under influent total nitrogen of 15 mg/L and COD/NO of 3.

Rapid enrichment of AnAOB with a novel vermiculite/tourmaline modification technology for enhanced DEAMOX process.

The DEnitrifying AMmonium OXidation (DEAMOX) has been proven to be a promising process treating contaminated surface water containing ammonia and nitrate, while the enrichment of the slow-growing anammox bacteria (AnAOB) remains a challenge. In this study, a novel polyurethane-adhesion vermiculite/tourmaline (VTP) modified carrier was developed to achieve effective enrichment of AnAOB. The results demonstrated that the VTP-1 (vermiculite: tourmaline = 1:1) system exhibited the greatest performance with the total nitrogen removal efficiency reaching 87.

Effects of soil colloids on adsorption and migration of benzo(a)pyrene on contaminated sites under runoff infiltration processes.

In the environment, soil colloids are widespread and possess a significant adsorption capacity. This makes them capable of transporting different pollutants, presenting a potential risk to human and ecological well-being. This study aimed to examine the adsorption and co-migration characteristics of benzo(a)pyrene (BaP) and soil colloids in areas contaminated with organic substances, utilizing both static and dynamic batch experiments.

The endocannabinoid system is involved in the anxiety-like behavior induced by dual-frequency 2.65/0.8 GHz electromagnetic radiation in mice.

As wireless communication devices gain popularity, concerns about the potential risks of environmental exposure to complex frequency electromagnetic radiation (EMR) on mental health have become a public health issue. Historically, EMR research has predominantly focused on single- frequency electromagnetic waves, neglecting the study of multi-frequency electromagnetic waves, which more accurately represent everyday life. To address these concerns, our study compared the emotional effects of single-frequency and dual-frequency EMR while exploring potential molecular mechanisms and intervention targets.

Bioremediation of 2,4,6-trichlorophenol by extracellular enzymes of white rot fungi immobilized with sodium alginate/hydroxyapatite/chitosan microspheres.

Hydroxyapatite, a calcium phosphate biomass material known for its excellent biocompatibility, holds promising applications in water, soil, and air treatment. Sodium alginate/hydroxyapatite/chitosan (SA-HA-CS) microspheres were synthesized by cross-linking sodium alginate with calcium chloride. These microspheres were carriers for immobilizing extracellular crude enzymes from white rot fungi through adsorption, facilitating the degradation of 2,4,6-trichlorophenol (2,4,6-TCP) in water and soil.

Recognizing Functional Groups of MES/APG Mixed Surfactants for Enhanced Solubilization toward Benzo[]pyrene.

Benzo[]pyrene is difficult to remove from soil due to its high octanol/water partition coefficient. The use of mixed surfactants can increase solubility but with the risk of secondary soil contamination, and the compounding mechanism is still unclear. This study introduced a new approach using environmentally friendly fatty acid methyl ester sulfonate (MES) and alkyl polyglucoside (APG) to solubilize benzo[]pyrene.

BLA DBS improves anxiety and fear by correcting weakened synaptic transmission from BLA to adBNST and CeL in a mouse model of foot shock.

Deep brain stimulation (DBS) in the basal lateral amygdala (BLA) has been established to correct symptoms of refractory post-traumatic stress disorder (PTSD). However, how BLA DBS operates in correcting PTSD symptoms and how the BLA elicits pathological fear and anxiety in PTSD remain unclear. Here, we discover that excitatory synaptic transmission from the BLA projection neurons (PNs) to the adBNST, and lateral central amygdala (CeL) is greatly suppressed in a mouse PTSD model induced by foot shock (FS).

Metagenomic insights into carbon and nitrogen cycling in the water-land transition zone of inland alkaline wetlands.

Inland alkaline wetlands play a crucial role in maintaining ecological functions. However, these wetlands are becoming more vulnerable to the effects of water level fluctuations caused by global climate change, especially concerning carbon (C) and nitrogen (N) cycling. Here, metagenomics sequencing was used to investigate microorganism diversity, C and N cycling gene abundance at three water level types (D (dry), MF (middle flooded), HF (high flooded)) along an inland alkaline wetland.

Mulches assist degraded soil recovery via stimulating biogeochemical cycling: metagenomic analysis.

Soil degradation of urban greening has caused soil fertility loss and soil organic carbon depletion. Organic mulches are made from natural origin materials, and represent a cost-effective and environment-friendly remediation method for urban greening. To reveal the effects of organic mulch on soil physicochemical characteristics and fertility, we selected a site that was covered with organic mulch for 6 years and a nearby lawn-covered site.

Bioremediation of Diesel-Contaminated Soil by Fungal Solid-State Fermentation.

To address the poor removal of diesel in soil by indigenous microorganisms, we proposed a fungal solid-state fermentation (SSF) method for bioremediation. We screened Pycnoporus sanguineus 5.815, Trametes versicolor 5.

Zeolite enhanced iron-modified biocarrier drives Fe(II)/Fe(III) cycle to achieve nitrogen removal from eutrophic water.

The problem of nitrogen removal in eutrophic water needs to be solved. Two new autotrophic nitrogen removal technologies, ammonia oxidation coupled with Fe(III) reduction (Feammox) and Nitrate-dependent Fe(II) oxidation (NDFO), have been shown to have the potential to treat eutrophic water. However, the continuous addition of iron sources not only costs more, but also leads to sludge mineralization.

Distinct laccase expression and activity profiles of facilitate degradation of benzo[a]pyrene.

A isolate from the Changbai Mountain showed promising activity in degrading benzo[a]pyrene (BaP), which is a high molecular weight (HMW) polycyclic aromatic hydrocarbon (PAH) compound. It was hypothesized that the isolate encode BaP-degrading enzymes, among which laccase is mostly sought after due to significant commercial potential. Genome of the isolate was sequenced and assembled, and seven laccase homologues were identified () as candidate genes potentially contributing to BaP degradation.

Effects of salinity on methane emissions and methanogenic archaeal communities in different habitat of saline-alkali wetlands.

The increase in temperature caused by global climate change has promoted the salinization of wetlands. Inland saline-alkaline wetlands have an environment of over-humidity and shallow water and are hot spots for CH emissions. However, there are few reports on the effect of salinity on CH emissions in inland saline-alkaline wetlands.

Greenhouse gas fluxes from different types of permafrost regions in the Daxing'an Mountains, Northeast China.

Global warming will increase the greenhouse gas (GHG) fluxes of permafrost regions. However, little is known about the difference in GHG fluxes among different types of permafrost regions. In this study, we used the static opaque chamber and gas chromatography techniques to determine the fluxes of carbon dioxide (CO), methane (CH), and nitrous oxide (NO) in predominantly continuous permafrost (PCP), predominantly continuous and island permafrost (PCIP), and sparsely island permafrost (SIP) regions during the growing season.

The biochar/Fe-modified biocarrier driven simultaneous NDFO and Feammox to remove nitrogen from eutrophic water.

Novelty techniques of Fe(III) reduction coupled to anaerobic ammonium oxidation (i.e. Feammox) and nitrate-dependent Fe(II) oxidation (i.