Bone formation of demineralized human dentin block graft with different demineralization time: In vitro and in vivo study.

Objective: To evaluate structural and physicochemical characteristics of demineralized human dentin block with increasing demineralization time and to assess new bone formation when onlay grafted at different demineralization times in rat calvaria.

Study Design: Extracted human permanent teeth were pretreated and demineralized for 10-90 min. Scanning electron microscopy, X-ray diffraction analysis, inductively coupled plasma spectrometry, and energy dispersive X-ray analysis were performed.

Demineralized deciduous tooth as a source of bone graft material: its biological and physicochemical characteristics.

Objective: To examine structural and physicochemical characteristics of demineralized deciduous tooth powder (DDTP) in relation to demineralization time and to present potential of using DDTP as a bone graft material.

Study Design: For structural and physicochemical analysis, scanning electron microscopy, inductively coupled plasma spectrometry, energy-dispersive X-ray analysis, X-ray diffraction analysis, differential scanning calorimetry, and Brunauer-Emmett-Teller surface area analysis were performed. In in vivo experiments, DDTP was grafted in 20 Sprague-Dawley rats' calvarial defects, and radiographic and histologic examination and histomorphometric analysis were performed.