Drug-Induced Differential Gene Expression Analysis on Nanoliter Droplet Microarrays: Enabling Tool for Functional Precision Oncology.

Drug-induced differential gene expression analysis (DGEA) is essential for uncovering the molecular basis of cell phenotypic changes and understanding individual tumor responses to anticancer drugs. Performing high throughput DGEA is challenging due to the high cost and labor-intensive multi-step sample preparation protocols. In particular, performing drug-induced DGEA on cancer cells derived from patient biopsies is even more challenging due to the scarcity of available cells.

Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays.

The aim of this study was to observe the effects of strophanthin induced inhibition of the Na-/K-ATPase in liver cells using a magnetic resonance (MR) compatible bioreactor. A microcavity array with a high density three-dimensional cell culture served as a functional magnetic resonance imaging (MRI) phantom for sodium multi quantum (MQ) spectroscopy. Direct contrast enhanced (DCE) MRI revealed the homogenous distribution of biochemical substances inside the bioreactor.

Results from a phantom based multi-centre paediatric computed tomography dose survey.

A computed tomography radiation dose survey was performed within our enterprise using three age-based paediatric phantoms representing a 1, 5 and 10 years old. Twenty-seven scanners were surveyed with volume computed tomography dose index and dose length product data collected for head, chest and abdomen-pelvis protocols at each age. Reconstruction method e.

Radiation dose in coronary angiography and intervention: initial results from the establishment of a multi-centre diagnostic reference level in Queensland public hospitals.

Introduction: Radiation dose to patients undergoing invasive coronary angiography (ICA) is relatively high. Guidelines suggest that a local benchmark or diagnostic reference level (DRL) be established for these procedures. This study sought to create a DRL for ICA procedures in Queensland public hospitals.

Exposure (mAs) optimisation of a multi-detector CT protocol for hepatic lesion detection: Are thinner slices better?

Introduction: The purpose of this work was to determine the exposure-optimised slice thickness for hepatic lesion detection with CT.

Methods: A phantom containing spheres (diameter 9.5, 4.

Optimization of computed tomography protocols: limitations of a methodology employing a phantom with location-known opacities.

This study aimed to determine if phantom-based methodologies for optimization of hepatic lesion detection with computed tomography (CT) require randomization of lesion placement and inclusion of normal images. A phantom containing fixed opacities of varying size (diameters, 2.4, 4.

Diagnostic reference levels for mammography in BreastScreen Queensland.

Diagnostic reference levels assist in the optimisation of radiation exposure parameters within a medical imaging facility. As no Australian DRLs currently exist, radiation doses from mammography in BreastScreen Queensland are analysed. Program-based DRLs of 1.

What are the true costs of major trauma?

Background: This economic evaluation reports the results of a detailed study of the cost of major trauma treated at Princess Alexandra Hospital (PAH), Australia.

Methods: A bottom-up approach was used to collect and aggregate the direct and indirect costs generated by a sample of 30 inpatients treated for major trauma at PAH in 2004. Major trauma was defined as an admission for Multiple Significant Trauma with an Injury Severity Score>15.

Suggested baseline ranges for performance testing of the Fujifilm FCR Capsula XL computed radiography system.

Due to the difficulty in obtaining expected baseline values from the supplier for the performance testing of the Fujifilm FCR Capsula XL computed radiography system, performance test results from the acceptance testing of 42 Capsula systems were reviewed. Guidance ranges are provided for nine system performance tests which can be applied to future testing of calibrated systems.

Chip-based three-dimensional cell culture in perfused micro-bioreactors.

We have developed a chip-based cell culture system for the three-dimensional cultivation of cells. The chip is typically manufactured from non-biodegradable polymers, e.g.