Complete genome sequence of F12-21, a halotolerant bacterium with antibacterial properties isolated from a Big Bone Lick State Park salt spring.

F12-21 is a halotolerant bacterium isolated from a sulfur-enriched salt spring. F12-21 inhibits bacteria of human health interest and bacterial salt spring co-inhabitants. We report the genome of F12-21, with a predicted genome of 3.

Draft genome sequence of the moderately halophilic bacterium sp. BBL2006.

We present the draft genome sequence of sp. BBL2006, a moderately halophilic, gram positive bacterium isolated from a sulfidic salt spring in Big Bone Lick State Park, Boone County, Kentucky. The genome of sp.

Reliable transformation system for Microbotryum lychnidis-dioicae informed by genome and transcriptome project.

Microbotryum lychnidis-dioicae is a member of a species complex infecting host plants in the Caryophyllaceae. It is used as a model system in many areas of research, but attempts to make this organism tractable for reverse genetic approaches have not been fruitful. Here, we exploited the recently obtained genome sequence and transcriptome analysis to inform our design of constructs for use in Agrobacterium-mediated transformation techniques currently available for other fungi.

Inoculum size effect in dimorphic fungi: extracellular control of yeast-mycelium dimorphism in Ceratocystis ulmi.

We studied the inoculum size effect in Ceratocystis ulmi, the dimorphic fungus that causes Dutch elm disease. In a defined glucose-proline-salts medium, cells develop as budding yeasts when inoculated at > or = 10(6) spores per ml and as mycelia when inoculated at <10(6) spores per ml. The inoculum size effect was not influenced by inoculum spore type, age of the spores, temperature, pH, oxygen availability, trace metals, sulfur source, phosphorous source, or the concentration of glucose or proline.

Spatial and resource factors influencing high microbial diversity in soil.

To begin defining the key determinants that drive microbial community structure in soil, we examined 29 soil samples from four geographically distinct locations taken from the surface, vadose zone, and saturated subsurface using a small-subunit rRNA-based cloning approach. While microbial communities in low-carbon, saturated, subsurface soils showed dominance, microbial communities in low-carbon surface soils showed remarkably uniform distributions, and all species were equally abundant. Two diversity indices, the reciprocal of Simpson's index (1/D) and the log series index, effectively distinguished between the dominant and uniform diversity patterns.