Wistar rats immature testicular tissue vitrification and heterotopic grafting.

Objective: To evaluate the efficiency of two vitrification protocols for rat immature testicular tissue and heterotopic transplantation.

Methods: Twenty-four pre-pubertal Wistar rats were divided into three groups (n=8). After orchiectomy, testicular fragments (3mm) from Groups 1 and 2 were vitrified with different cryoprotectant concentration solutions, using sterile inoculation loops as support.

Osteogenic potential of a chalcone in a critical-size defect in rat calvaria bone.

Background: This study describes the bone formation stimulated by the application of a type of chalcone to critical-size defects in rat calvarial bone.

Material And Methods: Sixty female Wistar rats were divided into 6 groups of 10 animals per group: control (no treatment), vehicle (vaseline) and the chalcone (1-phenyl-3-(4-chlorophenyl)-2-propen-1-one) suspended in vaseline at 10%. A critical-size defect of 5 mm was prepared using a trephine in the calvarial bone, after which the treatment was applied, in a single dose, according to the experimental group.

Development of tooth germ heterotopically grafted within the ear skin. An histological study in the rat.

The main goal of this study was the analysis of the developmental potentiality of tooth germ from late bell stage on, after its heterotopic placement within the skin. Teeth germs of newborn rats were grafted within a skin pouch of the ear of adult rats. Seven to fourteen days after grafting, dental germs developed normal dental structures in which ameloblasts and odontoblasts were well differentiated.