The TRPA1 Agonist Cinnamaldehyde Induces the Secretion of HCO by the Porcine Colon.

A therapeutic potential of the TRPA1 channel agonist cinnamaldehyde for use in inflammatory bowel disease is emerging, but the mechanisms are unclear. Semi-quantitative qPCR of various parts of the porcine gastrointestinal tract showed that mRNA for TRPA1 was highest in the colonic mucosa. In Ussing chambers, 1 mmol·L cinnamaldehyde induced increases in short circuit current (ΔI) and conductance (ΔG) across the colon that were higher than those across the jejunum or after 1 mmol·L thymol.

TRPV3 and TRPV4 as candidate proteins for intestinal ammonium absorption.

Aim: Absorption of ammonia from the gut has consequences that range from encephalitis in hepatic disease to global climate change induced by nitrogenous excretions from livestock. Since patch clamp data show that certain members of the transient receptor potential (TRP) family are permeable to NH , participation in ammonium efflux was investigated.

Methods: Digesta, mucosa and muscular samples from stomach, duodenum, jejunum, ileum, caecum and colon of pigs were analysed via colourimetry, qPCR, Western blot, immunohistochemistry and Ussing chambers.