Elucidating yeast glycolytic dynamics at steady state growth and glucose pulses through kinetic metabolic modeling.

Microbial cell factories face changing environments during industrial fermentations. Kinetic metabolic models enable the simulation of the dynamic metabolic response to these perturbations, but their development is challenging due to model complexity and experimental data requirements. An example of this is the well-established microbial cell factory Saccharomyces cerevisiae, for which no consensus kinetic model of central metabolism has been developed and implemented in industry.

Using Kinetic Modelling to Infer Adaptations in Carbohydrate Storage Metabolism to Dynamic Substrate Conditions.

Microbial metabolism is strongly dependent on the environmental conditions. While these can be well controlled under laboratory conditions, large-scale bioreactors are characterized by inhomogeneities and consequently dynamic conditions for the organisms. How response to frequent perturbations in industrial bioreactors is still not understood mechanistically.

pH dependencies of glycolytic enzymes of yeast under in vivo-like assay conditions.

Under carbon source transitions, the intracellular pH of Saccharomyces cerevisiae is subject to change. Dynamics in pH modulate the activity of the glycolytic enzymes, resulting in a change in glycolytic flux and ultimately cell growth. To understand how pH affects the global behavior of glycolysis and ethanol fermentation, we measured the activity of the glycolytic and fermentative enzymes in S.

Kinetic Modeling of Central Carbon Metabolism: Achievements, Limitations, and Opportunities.

Central carbon metabolism comprises the metabolic pathways in the cell that process nutrients into energy, building blocks and byproducts. To unravel the regulation of this network upon glucose perturbation, several metabolic models have been developed for the microorganism . These dynamic representations have focused on glycolysis and answered multiple research questions, but no commonly applicable model has been presented.