Publications by authors named "David K Y Lim"

Article Synopsis
  • Researchers studied Tetraselmis sp. M8 to understand how lipid pathways contribute to rapid triacylglyceride buildup after nitrogen exhaustion.
  • RNA-Seq and real-time PCR showed a significant change in gene expression related to fatty acid/triacylglyceride production and breakdown during different growth phases.
  • The findings suggest that increased lipid accumulation occurs due to reduced breakdown of lipids and enhanced production during the stationary phase, independent of the DGAT gene, which is typically important for lipid storage.
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With the depletion of global fish stocks, caused by high demand and effective fishing techniques, alternative sources for long chain omega-3 fatty acids are required for human nutrition and aquaculture feeds. Recent research has focused on land-based cultivation of microalgae, the primary producers of omega-3 fatty acids in the marine food web. The effect of salinity on fatty acids and related gene expression was studied in the model marine microalga, Tetraselmis sp.

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Omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provide significant health benefits and this has led to an increased consumption as dietary supplements. Omega-3 fatty acids EPA and DHA are found in animals, transgenic plants, fungi and many microorganisms but are typically extracted from fatty fish, putting additional pressures on global fish stocks. As primary producers, many marine microalgae are rich in EPA (C20:5) and DHA (C22:6) and present a promising source of omega-3 fatty acids.

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Microalgae have been widely reported as a promising source of biofuels, mainly based on their high areal productivity of biomass and lipids as triacylglycerides and the possibility for cultivation on non-arable land. The isolation and selection of suitable strains that are robust and display high growth and lipid accumulation rates is an important prerequisite for their successful cultivation as a bioenergy source, a process that can be compared to the initial selection and domestication of agricultural crops. We developed standard protocols for the isolation and cultivation for a range of marine and brackish microalgae.

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