The influence of adherent cell morphology on hydrodynamic recruitment of leukocytes.

Innate immunity is characterized by the coordinated activity of multiple leukocytes mobilizing at or near the site of tissue injury. Slow rolling and/or adherent leukocytes have been shown to hydrodynamically recruit free-stream leukocytes to a model of inflamed tissue. In this paper, we numerically investigate the hydrodynamic recruitment of free-stream leukocytes due to the presence of a nearby adherent, deformed leukocyte by using a computational model developed from first principles to simulate these types of interactions.

Shape oscillations of elastic particles in shear flow.

Particle suspensions are common to biological fluid flows; for example, flow of red- and white-blood cells, and platelets. In medical technology, current and proposed methods for drug delivery use membrane-bounded liquid capsules for transport via the microcirculation. In this paper, we consider a 3D linear elastic particle inserted into a Newtonian fluid and investigate the time-dependent deformation using a numerical simulation.

Deformable cell-cell and cell-substrate interactions in semi-infinite domain.

Leukocyte trafficking in the microvasculature during inflammatory response is known to involve multiple adhesion molecules and is referred to as the leukocyte adhesion cascade (LAC). Surface-bound selectins and their respective ligands are primarily responsible for tethering and rolling of leukocytes over inflamed endothelium. Numerical modeling of this response is challenging due to the nature of cell-cell interactions in Stokes flow (i.

Dimethylsulfoxide exposure modulates HL-60 cell rolling interactions.

Human leukaemic HL-60 cells are widely used for studying interactions involving adhesion molecules [e.g. P-selectin and PSGL-1 (P-selectin glycoprotein ligand-1)] since their rolling behaviour has been shown to mimic the dynamics of leucocyte rolling in vitro.