Publications by authors named "David I Ruby"
Article Synopsis
- Benzoylformate decarboxylase (BFDC) is a ThDP-dependent enzyme that plays a key role in the mandelate pathway and demonstrates a wide range of substrate specificity, making it valuable for creating chiral alpha-hydroxy ketones.
- The reaction between BFDC and methyl benzoylphosphonate (MBP) produces a stable analogue of the ThDP-substrate adduct, confirmed through circular dichroism and high-resolution mass spectrometry.
- The structure of BFDC with the MBP inhibitor has been determined via X-ray crystallography, revealing a tetrahedral adduct and providing insights into the reaction mechanism and comparisons to other ThDP-dependent enzymes.
