Morphometric analysis of myocardial mitochondria in rats during adaptation to exercise.

Comparative morphometric studies of the mitochondria of muscle cells and of interstitial cells of rat hearts were carried out in order to obtain more information about changes in mitochondrial parameters during adaptation of the myocardium to physical training of different durations (45, 180, 360 hours of swimming) and intensities. On the basis of the results the authors conclude that the mitochondrial biogenesis of heart muscle cells occurs in dependence on the intensity of training at two morphometrically different stages. Some parameters of the mitochondria of the interstitial cells reacted in a manner similar to that of the muscle cells, but opposite reactions were also observed.

Mutations affecting pigment synthesis in Mycobacterium aurum.

Pigmentation mutants of Mycobacterium aurum were isolated after chemical mutagenesis. Examination of the pigments extracted from these mutants indicated that at least 15 carotenoids were formed. beta-Carotene was not detected and the major carotene of M.

[Evaluation of urease and beta-glucosidase activity for the practical identification of mycobacteria (author's transl)].

The urease and the beta-glucosidase for the identification of the mycobacteria were evaluated using 1,019 strains received for reference purposes in this laboratory in 1978. We found that the urease test was useful in the differential identification of BCG which, contrary to Mycobacterium bovis, gave a strong positive reaction in 3 h. The test was also useful to discriminate M.

Evaluation of the chloride and bromide salts of cetylpyridium for the transportation of sputum in tuberculosis bacteriology.

The usefulness of cetylpyridium chloride and cetylpyridium bromide, at a final concentration of 1% in 1% solution of sodium chloride, for the transportation of sputum in tuberculosis bacteriology, was evaluated. It was found that these compounds did not alter the staining properties nor the viability of the tubercle bacilli for at least 14 days.

[Alterations of the volume and surface of liver cells of male rats during postnatal development].

To characterize the postnatal development of hepatocytes of male rats quantitative, morphometrical investigations of male rat livers were carried out at the following times: day of birth, 1st, 2nd, 3rd, 4th, 5th, 6th, 7th, 14th, 21st, and 28th day as well as 2nd, 3rd, 4th, 5th, and 6th month. Several methods were used to determine the cell volumes and surfaces as a whole, the nucleus, and the sinusoidal (supranuclear), lateral, and bile cancliculi-near cellular zone, as well as the sinusoidal, lateral, and bile canaliculi-near plasma membrane. The cellular volume increases from the day of birth (5,775.

Quantitative and qualitative changes in the mitochondria in hepatocytes during postnatal development of male rats.

The volume density of mitochondria increases during postnatal development of the hepatocytes of male rats (test ages: 0 [birth], 1st, 2nd, 3rd, 4th, 5th, 6th, 7th, 14th, 21st, 28th day and 2nd, 3rd, 4th, 5th and 6th month) from 0.165 at birth to 0.236 on the 14th day, after which it decreases to 0.

Some recent results of the quantitative characterization of heart muscle cells.

The present situation of the quantitative characterization of the heart muscle may be summarized as follows: 1. There exists a great collection of quantitative data of the heart at the macroscopical, microscopical, and ultrastructural levels. 2.

[Mycobacterium fortuitum infection after total hip prosthesis. A report of 3 cases (author's transl)].

The authors have observed three instances of sepsis due to Mycobacterium fortuitum complicating total hip replacement for osteoarthritis. The case histories are fully described. The requirements are given for recognition of the organism which grows on special culture media and which may be mistaken for Mycobacterium tuberculosis.

Interaction of Mycobacterium leprae and mycobacteriophage D29.

This study of the interaction between Mycobacterium leprae and the mycobacteriophage D29 showed that the viruses caused a patchy damage of cell wall structure and the accumulation in the host of internal crystalline structures. Whether the observed ultrastructural alterations were caused by the replication of D29 was not clear. Mitomycin C also caused the accumulation of crystalline structures in M.

Differentiation of mycobacteria by "in vivo" and "in vitro" delayed hypersensitivity reactions.

Guinea pigs were sensitized with heat killed M. tuberculosis, M. kansasii, M.

[Ultrastructural and functional changes of rabbit myocardium after cholesterol feeding].

The myocardium was ultrastructurally investigated immediately after 12 weeks of feeding in one part of the animals and in the remaining animals 27--30 months after termination of the feeding period. At the same time, parameters of the myocardial contraction were measured immediately after the feeding at isolated trabeculae of the left ventricle and 27--30 months after the termination of the feeding at the left ventricle in situ. After the 12 weeks of feeding the morphometrically estimated intracellular lipid content rises from 0.

[Reperfusion of the myocardium after acute ischemic lesion].

In a complex experimental study on 52 dogs the myocardium was examined after ischemia and reperfusion of various duration. Morphologic, metabolic, and functional findings proved that a short-term reperfusion after coronary occlusion for more than 2 hours has disadvantageous effects on the regression of ischemic lesions. During the early period (up to 3 days) this is caused by the "no-reflow" phenomenon.

Quantitative ultrastructural characterisation of rabbit erythroblasts and of 3H uridine incorporation into their nuclear components.

Electron microscopic-autoradiographic and morphometric investigations were carried out in erythroblasts from rabbit bone marrow. 1. to obtain quantitative data for the erythroblast classes.

[Experimental studies on surgical therapy of acute infarct].

The authors examined the effects of reperfusion after temporary ischemia in 50 dogs. The morphologic alterations were documented by methods of electron microscopy, fluorescence microscopy, and histochemistry. Lactate and activity of glycogen phosphorylase were assessed.