A genotyping array for the globally invasive vector mosquito, Aedes albopictus.

Background: Although whole-genome sequencing (WGS) is the preferred genotyping method for most genomic analyses, limitations are often experienced when studying genomes characterized by a high percentage of repetitive elements, high linkage, and recombination deserts. The Asian tiger mosquito (Aedes albopictus), for example, has a genome comprising up to 72% repetitive elements, and therefore we set out to develop a single-nucleotide polymorphism (SNP) chip to be more cost-effective. Aedes albopictus is an invasive species originating from Southeast Asia that has recently spread around the world and is a vector for many human diseases.

Papers published in Zootaxa concerning Neuropterida.

In the first twenty years of the publication of Zootaxa, more than two hundred papers have appeared in this journal that address the Neuropterida, which collectively includes the [Neuroptera+Raphidioptera]+Megaloptera sections (initially "Minor orders" section). A dozen submitted manuscripts were rejected before the review process, and another dozen submissions were rejected following the peer review process. Additional content and general submission history of these contributions is summarized here.

First record of the family Sialidae (Megaloptera) from Thailand and description of the female and putative larva of Indosialis bannaensis.

Indosialis bannaensis is reported from northern Thailand marking the first record of the family, genus, and species from this country. The female and the probable larva of the species are described for the first time.

Gynomorphic mandible morphology in the dobsonfly, Corydalus cornutus.

Two aberrant males of Corydalus cornutus (L.) (Insecta: Megaloptera), which exhibit unusually short mandibles with discrete dentition, are recorded from a locality in Missouri. Morphological details of the specimens, as well as implications for the overall morphological variation of the genus and species are discussed.

Preliminary survey of the mayflies (Ephemeroptera) and caddisflies (Trichoptera) of Big Bend Ranch State Park and Big Bend National Park.

The mayfly (Insecta: Ephemeroptera) and caddisfly (Insecta: Trichoptera) fauna of Big Bend National Park and Big Bend Ranch State Park are reported based upon numerous records. For mayflies, sixteen species representing four families and twelve genera are reported. By comparison, thirty-five species of caddisflies were collected during this study representing seventeen genera and nine families.

Identification of Aedes aegypti and its respective life stages by real-time polymerase chain reaction.

An Aedes aegypti-specific, fluorogenic probe hydrolysis (Taq-Man), polymerase chain reaction assay was developed for real-time screening using a field-deployable thermocycler. Laboratory-based testing of A. aegypti, A.

Rapid identification of dengue virus by reverse transcription-polymerase chain reaction using field-deployable instrumentation.

Dengue virus universal and dengue serotype 1 to 4, fluorogenic probe hydrolysis (TaqMan), reverse transcription-polymerase chain reaction assays were developed for screening and serotype identification of infected mosquito vectors and human sera using a field-deployable, fluorometric thermocycler. Dengue universal and dengue 1 to 4 serotype assay in vitro sensitivity and specificity results were 100% concordant when tested with total nucleic acid extracts of multiple strains of dengue serotype 1 to 4, yellow fever, Japanese encephalitis, West Nile, and St. Louis encephalitis viruses.