Serosurvey for Influenza D Virus Exposure in Cattle, United States, 2014-2015.

Influenza D virus has been detected predominantly in cattle from several countries. In the United States, regional and state seropositive rates for influenza D have previously been reported, but little information exists to evaluate national seroprevalence. We performed a serosurveillance study with 1,992 bovine serum samples collected across the country in 2014 and 2015.

Exploration of veterinary shortages in the wake of the Veterinary Feed Directive.

OBJECTIVE To identify geographic areas in the United States where food animal veterinary services may be insufficient to meet increased needs associated with the US FDA's Veterinary Feed Directive. DESIGN Cross-sectional study. SAMPLE Data collected between 2010 and 2016 from the US Veterinary Medicine Loan Repayment Program, the National Animal Health Monitoring System Small-Scale US Livestock Operations Study, and the USDA's National Veterinary Accreditation Program.

A survey of methods used for antimicrobial susceptibility testing in veterinary diagnostic laboratories in the United States.

Antimicrobial resistance is a serious threat to animal and human health worldwide, requiring a collaborative, holistic approach. The U.S.

Bovine leukemia virus seroprevalence among cattle presented for slaughter in the United States.

Infection with bovine leukemia virus (BLV) results in economic loss because of reduced productivity, especially reduced milk production, and early culling. In the United States, studies in 1996, 1999, and 2007 showed BLV infection to be widespread, especially in dairy herds. We updated information herein on BLV seroprevalence in the United States, using samples submitted for testing and found negative for antibodies for Brucella by the Kentucky Eastern Regional Federal Brucellosis Laboratory.

A serosurvey for ruminant pestivirus exposure conducted using cattle sera collected for brucellosis surveillance in the United States.

Four species of ruminant pestivirus are currently circulating in the United States: Bovine viral diarrhea virus 1 and 2 (BVDV-1, -2; predominant host: cattle), Border disease virus (BDV; predominant host: sheep), and pronghorn virus (sporadically detected in wild ruminants). A third bovine pestivirus called HoBi-like virus has been detected in cattle and water buffalo in South America, Asia, and Europe. To date, no isolations of HoBi-like viruses from U.

Case Series of Turkey Farms from the H5N2 Highly Pathogenic Avian Influenza Outbreak in the United States During 2015.

Between December 2014 and June 2015, an outbreak of H5N2 HPAI caused the largest and most expensive agriculture emergency in U.S. Department of Agriculture-Animal and Plant Health Inspection Service history.

The effects of omeprazole therapy on bacterial colonization of the pharynx in healthy dogs.

Objective: To identify the relative abundance of commensal pharyngeal flora in healthy dogs and determine if abundance of pharyngeal flora is altered during omeprazole administration.

Animals: Eight adult Beagles.

Procedures: A total of 3 baseline pharyngeal swabs, collected 48 hours apart, were obtained from each dog.

Salmonella Prevalence and Antimicrobial Susceptibility from the National Animal Health Monitoring System Sheep 2011 Study.

Salmonella is a major cause of foodborne illness and can cause clinical disease in animals. Understanding the on-farm ecology of Salmonella will be helpful in decreasing the risk of foodborne transmission. An objective of this study was to determine the prevalence of Salmonella among fecal samples collected on sheep operations in the United States.

Effectiveness of current anthelmintic treatment programs on reducing fecal egg counts in United States cow-calf operations.

During the United States Department of Agriculture (USDA) National Animal Health Monitoring System's (NAHMS) 2007-2008 beef study, producers from 24 states were offered the opportunity to evaluate their animals for internal parasites and for overall responses to treatment with anthelmintics. A lapse of 45 d was required between initial sampling and any previous treatments. Choice of anthelmintic (oral benzimidazoles, and both injectable and pour-on endectocides) was at the discretion of the producer so as not to alter the local control programs.

Prevalence of internal parasites in beef cows in the United States: Results of the National Animal Health Monitoring System's (NAHMS) beef study, 2007-2008.

During the United States Department of Agriculture (USDA) National Animal Health Monitoring System's (NAHMS) 2007-2008 beef study, 567 producers from 24 US States were offered the opportunity to collect fecal samples from weaned beef calves and have them evaluated for the presence of parasite eggs (Phase 1). Participating producers were provided with instructions and materials for sample collection. Up to 20 fresh fecal samples were collected from each of the 99 participating operations.

Summary of BRD data from the 2011 NAHMS feedlot and dairy heifer studies.

The USDA:APHIS National Animal Health Monitoring System collects data on health and health management in livestock and poultry populations throughout the USA in order to provide stakeholders with population estimates to use as benchmarks for comparison, to guide policy development, and to identify research needs and prioritize education efforts. Recent studies of both the beef cattle feedlot industry and dairy heifer rearing operations provided information about BRD occurrence as well as information about prevention and treatment practices used on these operations. While a great deal of effort is dedicated to BRD prevention, there are still opportunities to improve the strategies used.

Management practices associated with the rate of respiratory tract disease among preweaned beef calves in cow-calf operations in the United States.

Objective: To assess associations between herd management practices and herd-level rates of bovine respiratory disease complex (BRDC) in preweaned beef calves in US cow-calf operations.

Design: Cross-sectional survey.

Sample: 443 herds weighted to represent the US cow-calf population.

An investigation into beef calf mortality on five high-altitude ranches that selected sires with low pulmonary arterial pressures for over 20 years.

Producer reports from ranches over 2,438 meters in southwest Colorado suggest that the mortality of preweaned beef calves may be substantially higher than the national average despite the selection of low pulmonary pressure herd sires for over 20 years. Diagnostic investigations of this death loss problem have been limited due to the extensive mountainous terrain over which these calves are grazed with their dams. The objective of the current study was to determine the causes of calf mortality on 5 high-altitude ranches in Colorado that have been selectively breeding sires with low pulmonary pressure (<45 mmHg) for over 20 years.

Prevalence of Escherichia coli O-types and Shiga toxin genes in fecal samples from feedlot cattle.

While efforts to control foodborne illness associated with the Shiga toxin-producing Escherichia coli (E. coli) O157 through processes and procedures implemented at harvest facilities have been very successful, there is concern about the burden of illness associated with other Shiga toxin-producing E. coli.

Farm-level associations with the shedding of Salmonella and antimicrobial-resistant Salmonella in U.S. dairy cattle.

Salmonella enterica is the leading cause of foodborne-related deaths and hospitalizations within the United States. Infections caused by antimicrobial-resistant (AMR) strains are associated with higher hospital costs and case fatality. The objective for this study was to determine the association of management practices with the recovery of Salmonella and AMR Salmonella on dairy herds.

Prevalence and genotypes of Enterocytozoon bieneusi in weaned beef calves on cow-calf operations in the USA.

To determine the prevalence and genotype distribution of Enterocytozoon bieneusi in weaned beef calves in the USA, fecal samples were collected from 819 calves (6-18 months of age) from 49 operations. Feces were sieved and subjected to density gradient centrifugation to remove fecal debris and to concentrate spores. DNA extracted from each sample was subjected to the polymerase chain reaction (PCR) to amplify the complete internal transcriber spacer (ITS).

Prevalence of Giardia duodenalis assemblages in weaned cattle on cow-calf operations in the United States.

To determine the prevalence of Giardia duodenalis in weaned beef calves on cow-calf operations in the United States, fecal specimens were collected from 819 calves (6-18 months of age) from 49 operations. After cleaning and concentration procedures to maximize recovery of cysts from feces, DNA was extracted from each of the 819 specimens. The presence of G.

Effects of restricted antimicrobial exposure on antimicrobial resistance in fecal Escherichia coli from feedlot cattle.

Objectives: The primary objective was to evaluate differences in antimicrobial resistance among enteric bacteria recovered from feedlot cattle that were being raised without exposure to antimicrobial drugs (AMDs) and those reared using conventional practices.

Materials: Forty pens of feedlot cattle (4557 total animals) that were being fed without AMD exposures were selected for enrollment as were 44 pens of cattle (4913 total animals) being fed for production of conventional beef products at the same feedlots. Fecal samples were collected from the floors of pens approximately biweekly through the middle of the feeding period and again prior to slaughter.

Species of Cryptosporidium detected in weaned cattle on cow-calf operations in the United States.

To determine the species and distribution of Cryptosporidium in weaned beef calves in the United States, fecal specimens were collected from 819 cattle between 6 and 18 months of age from 49 operations in 20 states (Alabama, California, Colorado, Georgia, Idaho, Iowa, Kansas, Louisiana, Mississippi, Missouri, Nebraska, New Mexico, North Dakota, Oklahoma, Oregon, South Dakota, Tennessee, Texas, Virginia, and Wyoming). Fresh feces, collected either from the ground or directly from the rectum of each animal, were sieved and subjected to density gradient centrifugation to remove fecal debris and to concentrate oocysts. DNA extracted from each specimen was subjected to the polymerase chain reaction (PCR) using primers for the SSU rRNA gene.

Analysis of risk factors associated with Salmonella spp. isolated from U.S. feedlot cattle.

Contamination can occur at a number of stages during farm-to-fork processing. Preharvest intervention is an ongoing area of interest in reduction of risk of foodborne illness. This study examined risk factors associated with detection of Salmonella from cattle in U.

Antimicrobial drug use and antimicrobial resistance in enteric bacteria among cattle from Alberta feedlots.

The purpose of this study was to determine whether antimicrobial resistance (AMR) in foodborne pathogens (Escherichia coli O157, Salmonella, and Campylobacter) and non-type-specific E. coli obtained from fecal samples of feedlot cattle was associated with antimicrobial drug (AMD) use. A secondary objective was to determine if AMR in non-type-specific E.

Occurrence of foodborne bacteria in Alberta feedlots.

The occurrence of generic Escherichia coli, E. coli O157, Salmonella, and Campylobacter in cattle manure, beef carcasses, catch basin water, and soils receiving manure application was determined in 21 Alberta feedlots. In cattle manure, generic E.

Relationship between herd size and annual prevalence of and primary antimicrobial treatments for common diseases on dairy operations in the United States.

Dairy operations in states representing 86% of the United States' national dairy herd were surveyed regarding the occurrence of common dairy diseases or disorders (digestive, respiratory, gastrointestinal, lameness, mastitis, navel infection) in specified production groups (weaned heifers, unweaned heifers, adult cows), and the most common antimicrobial preparation used to treat these conditions. Within disease and production group, disease frequency and characteristics of primary antimicrobial (drug class, availability, spectrum of activity, extra-label usage, withdrawal times) were compared among herd sizes (30-99, 100-499, and >/=500 cows) using chi-square or t-tests that accounted for the sampling design. The most common diseases were mastitis and lameness in cows, and gastrointestinal disease in unweaned calves, affecting 16, 11, and 15% of the target populations, respectively.