Experimental validation of Na( γ, n)Na cross section.

Photonuclear reactions are gaining importance due to their influence on the shielding and activation of components of widely spread accelerators. Therefore, there is a need for accurate data describing photonuclear reactions. The MT25 microtron operated at NPI was used for validation of cross section in the bremsstrahlung.

Cellular uptake and fate of cationic polymer-coated nanodiamonds delivering siRNA: a mechanistic study.

Gene silencing using small interfering RNAs (siRNAs) is a selective and promising approach for treatment of numerous diseases. However, broad applications of siRNAs are compromised by their low stability in a biological environment and limited ability to penetrate cells. Nanodiamonds (NDs) coated with cationic polymers can enable cellular delivery of siRNAs.

The electron beam irradiation of collagen in the dry and gel states: The effect of the dose and water content from the primary to the quaternary levels.

The aim of our study was to describe the impact of collagen in the gel and dry state to various doses of electron beam radiation (1, 10 and 25 kGy) which are using for food processing and sterilization. The changes in the chemical compositions (water, amino acids, lipids, glycosaminoglycans) were analyzed and the changes in the structure (triple-helix or β-sheet, the integrity of the collagen) were assessed. Subsequently, the impact of the applied doses on the mechanical properties, stability in the enzymatic environment, swelling and morphology were determined.

COMPARISON OF OSL AND TL DOSEMETERS WITH DATA COLLECTED AT THE MT25 CYCLIC ELECTRON ACCELERATOR.

The Microtron MT25 is a cyclic electron accelerator with a Kapitza resonator, maximum beam energy of 25 MeV, standard repetition frequency of 423 Hz, pulse length of 3.5 μs and mean current of 30 μA. Studies at conventional particle accelerators allow to understand the response of dosemeters in known and controllable radiation fields.

Different Mechanisms of DNA Radiosensitization by 8-Bromoadenosine and 2'-Deoxy-2'-fluorocytidine Observed on DNA Origami Nanoframe Supports.

DNA origami nanoframes with two parallel DNA sequences are used to evaluate the effect of nucleoside substituents on radiation-induced DNA damage. Double strand breaks (DSB) of DNA are counted using atomic force microscopy (AFM), and total number of lesions is evaluated using real-time polymerase chain reaction (RT-PCR). Enhanced AT or GC content does not increase the number of DNA strand breaks.

Diamond nano-optode for fluorescent measurements of pH and temperature.

Nano-optodes with a diamond core coated with a double stimuli-responsive polymeric shell reversibly respond to pH and temperature changes. Swelling and collapsing of the shell are accompanied by changes in the charge of the polymer. Changes in the fluorescent spectra of nitrogen-vacancy centers ratiometrically indicate pH and temperature.

Modified glycogen as construction material for functional biomimetic microfibers.

We describe a conceptually new, microfibrous, biodegradable functional material prepared from a modified storage polysaccharide also present in humans (glycogen) showing strong potential as direct-contact dressing/interface material for wound healing. Double bonds were introduced into glycogen via allylation and were further exploited for crosslinking of the microfibers. Triple bonds were introduced by propargylation and served for further click functionalization of the microfibers with bioactive peptide.

Toward structured macroporous hydrogel composites: electron beam-initiated polymerization of layered cryogels.

The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer-Emmett-Teller surface area analysis, and stiffness measurements.