Publications by authors named "David Baruc Cruvinel Lima"
Article Synopsis
- Testicular vitrification is a method used to preserve the genetic material of pre-pubertal animals, but there’s limited research on how to effectively warm vitrified testicular tissues.
- This study assessed how different warming temperatures (50, 55, and 60°C) affect the integrity and viability of vitrified testicular fragments from pre-pubertal cats through histological and mitochondrial activity evaluations.
- Results indicated that warming at 50°C maintained tubular integrity and cell junctions better than higher temperatures, with 60°C causing significant damage, making 50°C the optimal temperature for preserving testicular morphology and function.
Systematic cryo-banking of semen and testicular tissues is critical to preserve the genetic value of recently deceased or neutered black-footed ferrets (BFFs). Specifically, recovering or producing mature sperm cells from vitrified-warmed issues offers additional options in assisted reproduction. This could, in turn, enhance the genetic management of this rare and endangered species over multiple generations.
View Article and Find Full Text PDFUnderstanding critical roles of warming and reanimation is critical to improve the survival of vitrified testicular tissue in domestic cats. The objective was to study structural and functional properties of testicular tissues from prepubertal domestic cats after standard vitrification followed by two warming protocols (directly at 37°C or with a 5-second pre-exposure to 50°C) and three reanimation time points (immediately, 24 h and 5 days post-warming). In Experiment 1, tissues were evaluated for histo-morphology and mitochondrial activity immediately or 24 h after warming protocols.
View Article and Find Full Text PDFProtocols for the cryopreservation of testicular tissue are not yet established. In cats, few studies have been conducted on testicular vitrification using different cryoprotectant associations (CPAs). Thus, the objective of this study was to compare the effect of different CPAs on the vitrification of testicular tissue from prepubertal cats in cryotubes.
View Article and Find Full Text PDFThe present study was conducted to identify the major proteome of the sperm-rich fraction and prostatic fraction of canine seminal plasma. Three semen samples from four healthy dogs were obtained by digital manipulation. The pre-sperm fraction, sperm-rich fraction and prostatic fraction were separated from each ejaculate.
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