Publications by authors named "David B Morse"
Article Synopsis
- Droplet microfluidic methods have improved the efficiency of single-cell sequencing but face challenges like increased background noise and lower RNA capture rates due to the lack of effective cell enrichment strategies.
- The presented methodology uses fluorescence-activated droplet sorting to isolate droplets containing viable or specific cell types and employs picoinjection for multi-step processes, enhancing gene detection by five times and reducing noise by up to 50%.
- This approach successfully creates a high-quality molecular atlas of mouse brain development and nascent RNA transcription during organogenesis, and can be adapted for various other droplet-based workflows to achieve cost-effective and precise single-cell profiling.
Single-cell RNA sequencing (scRNA-seq) is a powerful technique for describing cell states. Identifying the spatial arrangement of these states in tissues remains challenging, with the existing methods requiring niche methodologies and expertise. Here, we describe segmentation by exogenous perfusion (SEEP), a rapid and integrated method to link surface proximity and environment accessibility to transcriptional identity within three-dimensional (3D) disease models.
View Article and Find Full Text PDFPurpose: Chromosomal aberration and DNA copy number change are robust hallmarks of cancer. The gold standard for detecting copy number changes in tumor cells is fluorescence in situ hybridization (FISH) using locus-specific probes that are imaged as fluorescent spots. However, spot counting often does not perform well on solid tumor tissue sections due to partially represented or overlapping nuclei.
View Article and Find Full Text PDFMicroscale hydrogels consisting of macromolecular networks in aqueous continuous phases have received increasing attention because of their potential use in tissue engineering, cell encapsulation and for the storage and release of cargo molecules. However, for applications targeting intracellular delivery, their micrometer-scale size is unsuitable for effective cellular uptake. Nanoscale analogs of such materials are thus required for this key area.
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