Southern Hemisphere dominates recent decline in global water availability.

Global land water underpins livelihoods, socioeconomic development, and ecosystems. It remains unclear how water availability has changed in recent decades. Using an ensemble of observations, we quantified global land water availability over the past two decades.

Purification, cloning, and properties of alpha-galactosidase from Saccharopolyspora erythraea and its use as a reporter system.

An alpha-galactosidase from the erythromycin-producing bacterium Saccharopolyspora erythraea was purified to near homogeneity. The enzyme has an apparent molecular mass of 45 kDa as determined by SDS-PAGE. The pH optimum, K(m) for p-nitrophenyl-alpha-D: -glucopyranoside (pNPalphaG), K(m) for melibiose and the V(max) are similar to those of other studied alpha-galactosidase enzymes.

Physical-genetic map of the erythromycin-producing organism Saccharopolyspora erythraea.

A physical map of the chromosome of the erythromycin-producing actinomycete Saccharopolyspora erythraea NRRL 2338 has been constructed using the restriction enzymes AseI and DraI. The map was constructed by a variety of methods including linking clone analysis, cross-hybridizations using labelled macrorestriction fragments, gene probing, two-dimensional PFGE and restriction enzyme site generation. Analysis of the individual macrorestriction patterns of the 17 AseI-, 6 DraI- and 22 AseI/DraI-digested fragments indicated a chromosome size of about 8 Mb.

The defective phosphoribosyl diphosphate synthase in a temperature-sensitive prs-2 mutant of Escherichia coli is compensated by increased enzyme synthesis.

An Escherichia coli strain which is temperature-sensitive for growth due to a mutation (prs-2) causing a defective phosphoribosyl diphosphate (PRPP) synthase has been characterized. The temperature-sensitive mutation was mapped to a 276 bp HindIII-BssHII DNA fragment located within the open reading frame specifying the PRPP synthase polypeptide. Cloning and sequencing of the mutant allele revealed two mutations.