Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates.

Background: Specific productivity (q) in yeast correlates with growth, typically peaking at intermediate or maximum specific growth rates (μ). Understanding the factors limiting productivity at extremely low μ might reveal decoupling strategies, but knowledge of production dynamics and physiology in such conditions is scarce. Retentostats, a type of continuous cultivation, enable the well-controlled transition to near-zero µ through the combined retention of biomass and limited substrate supply.

Metabolic engineering of Pichia pastoris.

Besides its use for efficient production of recombinant proteins the methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to produce metabolites of varying origin.

Comprehensive assessment of measurement uncertainty in C-based metabolic flux experiments.

In the field of metabolic engineering C-based metabolic flux analysis experiments have proven successful in indicating points of action. As every step of this approach is affected by an inherent error, the aim of the present work is the comprehensive evaluation of factors contributing to the uncertainty of nonnaturally distributed C-isotopologue abundances as well as to the absolute flux value calculation. For this purpose, a previously published data set, analyzed in the course of a C labeling experiment studying glycolysis and the pentose phosphate pathway in a yeast cell factory, was used.

Systems biotechnology for protein production in Pichia pastoris.

The methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) is one of the most important production systems for heterologous proteins.

Hemiphiltrum rotated flap.

In three patients of different ages, large resections of the philtrum have been repaired by rotating island flaps from the residual philtrum, leaving inconspicuous scars and no contour deformity. The rotated hemiphiltrum flap described here is an easy and safe procedure performed with local anesthesia that may be an effective cosmetic and functional repair of selected difficult surgical defects of the central upper lip involving the philtrum.