Publications by authors named "David A Moraga Amador"
Exposure to contaminants, such as the herbicide glyphosate, can suppress protective immune functions. Glyphosate is the herbicide most used worldwide and has been found in the plasma of more than 50 % of the Florida manatees (Trichechus manatus latirostris) and all-year-round in their aquatic environment. Our objectives were to analyze the consequences of glyphosate exposure on their immune responses via T-lymphocyte proliferation assays and transcriptomics.
View Article and Find Full Text PDFKaposi's sarcoma-associated herpesvirus is the etiologic agent of Kaposi's sarcoma and two B-cell malignancies. Recent advancements in sequencing technologies have led to high resolution transcriptomes for several human herpesviruses that densely encode genes on both strands. However, for KSHV progress remained limited due to the overall low percentage of KSHV transcripts, even during lytic replication.
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- * They generated over 427 million long-read sequences and found that longer, more accurate sequences yield better transcript detection, while increased read depth enhances quantification.
- * The study suggests that using reference-based tools works best for well-annotated genomes and recommends incorporating extra data to better identify rare transcripts, providing a benchmark for improving transcriptome analysis techniques in the future.
Article Synopsis
- The Long-read RNA-Seq Genome Annotation Assessment Project (LRGASP) Consortium aimed to evaluate long-read sequencing for analyzing transcripts by generating over 427 million sequences from various species.
- The findings highlighted that longer, accurate sequences yield better transcript identification, while increased read depth enhances quantification accuracy, particularly in well-annotated genomes.
- The study serves as a benchmark for transcriptome analysis strategies and suggests using additional data for detecting rare transcripts or employing reference-free methods.
Article Synopsis
- Glyphosate, a widely-used herbicide, is utilized in agriculture and environments like sugar cane fields in Florida, but its runoff poses threats to aquatic wildlife and has been linked to health issues such as kidney and liver damage in animal studies.
- In a study, adult male largemouth bass were exposed to different concentrations of glyphosate and Rodeo® for 21 days, leading to significant reductions in steroid hormones, including androgens and estrone.
- RNA sequencing revealed that glyphosate exposure altered gene expression related to hormone biosynthesis and immune processes, indicating potential endocrine disruption and impacting T-cell activation in the bass.
Oil recovery is a challenge and microbial enhanced oil recovery is an option. We theorized that the use of produced water (PW) with photo-stimulation could influence both production and viscosity of Xanthan gum. This study aimed at the evaluation of the effect of photo-stimulation by λ630 ± 1 ηm LED light on the biosynthesis of Xanthan gum produced by Xanthomonas campestris IBSBF 2103 strain reusing PW of the oil industry.
View Article and Find Full Text PDFEffects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.
J Photochem Photobiol B
December 2020
Oil is expected to continue to be one of the most important sources of energy in the world and world's energy matrix for the foreseeable future. However, high demand for energy and the decline of the production of oil fields makes oil recovery a challenge. Most techniques used for the recovery process are expensive, non-sustainable and technically difficult to implement.
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