The Freshwater Cyanobacterium PCC 7942 Does Not Require an Active External Carbonic Anhydrase.

Under standard laboratory conditions, PCC 7942 lacks EcaA, a periplasmic carbonic anhydrase (CA). In this study, a transformant was created that expressed the homologous EcaA from sp. ATCC 51142.

Optimization of CO Supply for the Intensive Cultivation of IPPAS C-1 in the Laboratory and Pilot-Scale Flat-Panel Photobioreactors.

Microalgae are increasingly being used for capturing carbon dioxide and converting it into valuable metabolites and biologically active compounds on an industrial scale. The efficient production of microalgae biomass requires the optimization of resources, including CO. Here, we estimated the productivity of IPPAS C-1 depending on CO concentrations and the ventilation coefficient of the gas-air mixture (GAM) in flat-panel photobioreactors (FP-PBRs) at laboratory (5 L) and pilot (18 L) scales.

Cultivation of IPPAS C-1 in Flat-Panel Photobioreactors: From a Laboratory to a Pilot Scale.

Flat-panel photobioreactors are effective systems for microalgae cultivation. This paper presents the growth characteristics of the microalgae IPPAS C-1 as a result of three-stage scale-up cultivation in a specially designed cultivation system. First, was grown aseptically in 250 mL glass vessels; then, it was diluted and inoculated into a 5-liter flat-panel horizontal photobioreactor; and, at the last stage, the culture was diluted and inoculated into a 70-liter flat-panel vertical photobioreactor.

Highly active extracellular α-class carbonic anhydrase of Cyanothece sp. ATCC 51142.

Here, for the first time, we report the presence of highly active extracellular carbonic anhydrase (CA) of α-class in cyanobacterial cells. The enzyme activity was confirmed both in vivo in intact cells and in vitro, using the recombinant protein. CA activity in intact cells of Cyanothece sp.

Hydrogen Peroxide Participates in Perception and Transduction of Cold Stress Signal in Synechocystis.

The double mutant ΔkatG/tpx of cyanobacterium Synechocystis sp. strain PCC 6803, defective in the anti-oxidative enzymes catalase (KatG) and thioredoxin peroxidase (Tpx), is unable to grow in the presence of exogenous H2O2. The ΔkatG/tpx mutant is shown to be extremely sensitive to very low concentrations of H2O2, especially when intensified with cold stress.

Draft Genome Sequences of Two Thermotolerant Cyanobacterial Strains Isolated from Hot Springs.

We report here two draft cyanobacterial genome sequences, those of IPPAS B-1201, isolated from a hot spring in the Turgen Gorge (Kazakhstan), and the uncharacterized cyanobacterium IPPAS B1203, isolated from a hot spring in Karlovy Vary (Czech Republic). These two strains were deposited at the Collection of Microalgae (IPPAS) of the Timiryazev Institute of Plant Physiology.